Gryczynski Ignacy, Malicka Joanna, Lakowicz Joseph R, Goldys Ewa M, Calander Nils, Gryczynski Zygmunt
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.
Optical Microcharacterisation Facility, Division of Information and Communication Sciences, Macquarie University, Sydney, NSW 2109, Australia.
Thin Solid Films. 2005 Nov 22;491(1-2):173-176. doi: 10.1016/j.tsf.2005.06.010. Epub 2005 Jul 11.
We measured a directional surface plasmon-coupled emission (SPCE) induced by a two-photon absorption. A 60 nm thick layer of poly(vinyl alcohol) film doped with rhodamine 123 was deposited on a silvered (50 nm Ag) glass slide, which was attached to a hemicylindrical glass prism. The 820 nm excitation from a femtosecond Ti:Sapphire laser was used either in reverse Kretschmann or Kretschmann configuration. The angular distribution of two-photon induced SPCE does not depend on the used configuration. The two-photon induced SPCE can be applied to improve immunoassays and deoxyribonucleic acid detection.
我们测量了由双光子吸收诱导的定向表面等离子体耦合发射（SPCE）。将掺杂有罗丹明123的60纳米厚的聚乙烯醇薄膜层沉积在镀银（50纳米银）的载玻片上，该载玻片附着在半圆柱形玻璃棱镜上。使用飞秒钛宝石激光器的820纳米激发光，采用反向 Kretschmann 或 Kretschmann 配置。双光子诱导的SPCE的角分布不取决于所使用的配置。双光子诱导的SPCE可用于改进免疫测定和脱氧核糖核酸检测。
