Chen Chung-Yu, Yuen Hei-Man, Lin Chung-Chi, Hsu Chi-Chieh, Bernard Jeffrey R, Chen Ling-Ni, Liao Yi-Hung, Tsai Shiow-Chwen
Department of Exercise and Health Sciences, University of Taipei, Taipei City, Taiwan.
Institute of Sports Sciences, University of Taipei, Taipei City, Taiwan.
Front Physiol. 2021 Mar 22;12:651972. doi: 10.3389/fphys.2021.651972. eCollection 2021.
Fish soup is a traditional Chinese food usually offered as a healthy supplement to elders, pregnant women and persons who just had surgery. Silver perch (Santé premium silver perch essence, SPSPE) extract contains various quality proteins, collagen, minerals, trace elements, and branch chain amino acids (BCAA) that could help individuals recover from exhaustion and control body weight. However, there are very limited studies exploring the effects of fish extracts on exercise performance and fatigue, and relevant physiological mechanisms. Therefore, the purpose of this study was to investigate the effects of chronic SPSPE administration on exhaustive exercise performance. Male Wistar rats weighing around 250 g were divided into 4 groups: Control, 1X SPSPE (6.2 ml/kg), 2X SPSPE (12.4 ml/kg) and 5X SPSPE (31.0 ml/kg). Rats were administrated SPSPE by oral gavage feeding every day for 33 days. Their body weight were measured every week. Before and after the exhaustive swimming test, the blood was collected for circulating lactate, glucose, ammonia, hormones, and myoglobin analysis. Rats were sacrificed after performing an exhaustive swimming exercise test. The liver tissues were collected for glycogen content and H&E staining. After the administration of 1X and 5X SPSPE, swimming fatigue was significantly delayed ( = 0.024). There was no difference in the hormone plasma level between the control and SPSPE groups. The induction of plasma corticosterone and TBARS by exhaustive swimming exercise could be decreased by SPSPE administration. The increased plasma myoglobin concentration from exhaustive swimming exercise was weakened by SPSPE supplementation. The higher glycogen sparing contained in liver tissue was observed in SPSPE-treated groups ( < 0.05). SPSPE could efficiently delay swimming fatigue through sparing of liver glycogen and attenuation of plasma TBARS, myoglobin induction by exhaustive exercise. Our findings provide a scientific-based fundamental information and better understanding for developing a fish extract-based anti-fatigue supplement.
鱼汤是一种传统的中式食物，通常作为给老年人、孕妇和刚做完手术的人的健康补充品。银鲈（圣特优质银鲈精华，SPSPE）提取物含有多种优质蛋白质、胶原蛋白、矿物质、微量元素和支链氨基酸（BCAA），有助于个体从疲劳中恢复并控制体重。然而，探索鱼类提取物对运动表现和疲劳影响及其相关生理机制的研究非常有限。因此，本研究的目的是调查长期给予SPSPE对力竭运动表现的影响。体重约250克的雄性Wistar大鼠分为4组：对照组、1倍剂量SPSPE组（6.2毫升/千克）、2倍剂量SPSPE组（12.4毫升/千克）和5倍剂量SPSPE组（31.0毫升/千克）。大鼠每天通过灌胃给予SPSPE，持续33天。每周测量它们的体重。在力竭游泳试验前后，采集血液用于循环乳酸、葡萄糖、氨、激素和肌红蛋白分析。在进行力竭游泳运动试验后处死大鼠。收集肝脏组织用于糖原含量和苏木精-伊红染色分析。给予1倍剂量和5倍剂量SPSPE后，游泳疲劳显著延迟（P = 0.024）。对照组和SPSPE组之间血浆激素水平没有差异。给予SPSPE可降低力竭游泳运动诱导的血浆皮质酮和丙二醛水平。补充SPSPE可减弱力竭游泳运动导致的血浆肌红蛋白浓度升高。在SPSPE处理组中观察到肝脏组织中糖原储备更高（P < 0.05）。SPSPE可通过节省肝糖原和减轻力竭运动诱导的血浆丙二醛、肌红蛋白水平来有效延迟游泳疲劳。我们的研究结果为开发基于鱼类提取物的抗疲劳补充剂提供了基于科学的基础信息和更好的理解。
