Zou Xiaoxiao, Yokoyama Wallace, Liu Xiaohui, Wang Kai, Hong Hui, Luo Yongkang, Tan Yuqing
Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Healthy Processed Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Albany, CA 94710, USA.
Antioxidants (Basel). 2023 Mar 13;12(3):712. doi: 10.3390/antiox12030712.
Milk fat globule membranes (MFGMs) are complex structures that incorporate bioactive proteins and lipids to assist in infant development. However, the antifatigue and antioxidant potentials of MFGM have not been investigated. In this study, repeated force swimming measured fatigue in male BALB/c mice fed MFGM and saline for 18 weeks. The MFGM supplementation increased the time to exhaustion by 42.7% at 6 weeks and 30.6% at 14 weeks ( < 0.05). Fatigue and injury-related biomarkers, including blood glucose, lactic acid, and lactate dehydrogenase, were ameliorated after free swimming ( < 0.05). The activity of antioxidant enzymes in blood serum increased at 18 weeks, while malondialdehyde (MDA) content decreased by 45.0% after the MFGM supplementation ( < 0.05). The Pearson correlation analysis showed a high correlation between fatigue-related indices and antioxidant levels. The increased protein expression of hepatic Nrf2 reduced the protein expression of Caspase-3 in the gastrocnemius muscle ( < 0.05). Moreover, the MFGM supplementation increased the relative abundance of , , and . Our results demonstrate that MFGM may maintain redox homeostasis to relieve fatigue, suggesting the potential application of MFGM as an antifatigue and antioxidant dietary supplement.
乳脂肪球膜（MFGMs）是一种复杂的结构，其中包含生物活性蛋白质和脂质，有助于婴儿发育。然而，MFGM的抗疲劳和抗氧化潜力尚未得到研究。在本研究中，通过重复力竭游泳实验来测定喂食MFGM和生理盐水18周的雄性BALB/c小鼠的疲劳程度。补充MFGM后，6周时力竭时间增加了42.7%，14周时增加了30.6%（P<0.05）。自由游泳后，与疲劳和损伤相关的生物标志物，包括血糖、乳酸和乳酸脱氢酶，均有所改善（P<0.05）。血清中抗氧化酶的活性在18周时增加，而补充MFGM后丙二醛（MDA）含量降低了45.0%（P<0.05）。Pearson相关性分析表明，与疲劳相关的指标和抗氧化水平之间存在高度相关性。肝脏中Nrf2蛋白表达的增加降低了腓肠肌中Caspase-3的蛋白表达（P<0.05）。此外，补充MFGM增加了……、……和……的相对丰度。我们的结果表明，MFGM可能通过维持氧化还原稳态来缓解疲劳，这表明MFGM作为一种抗疲劳和抗氧化膳食补充剂具有潜在的应用价值。
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