Laboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, China.
Oxid Med Cell Longev. 2021 Mar 19;2021:5595376. doi: 10.1155/2021/5595376. eCollection 2021.
Artificial illumination may interfere with biological rhythms and distort physiological homeostasis in avian. Our previous study demonstrated that 660 nm red light exacerbates oxidative stress, but a combination of green and blue lights (G→B) can improve the antibody titer in chickens compared with single monochromatic light. Melatonin acts as an antioxidant which is a critical signaling to the coordination between external light stimulation and the cellular response from the body. This study further clarifies the potential role of melatonin in monochromatic light combination-induced bursa B-lymphocyte proliferation in chickens. A total of 192 chicks were exposed to a single monochromatic light (red (R), green (G), blue (B), or white (W) lights) or various monochromatic light combinations (B→G, G→B, and R→B) from P0 to P42. We used qRT-PCR, MTT, western blotting, immunohistochemistry, and Elisa to explore the effect of a combination of monochromatic light on bursa B-lymphocytes and its intracellular signal pathways. With consistency in the upregulation in melatonin level of plasma and antioxidant enzyme ability, we observed increases in organ index, follicle area, lymphocyte density, B-lymphocyte proliferation, PCNA-positive cells, and cyclin D1 expression in bursa of the G→B group compared with other light-treated groups. Melatonin bound to Mel1a and Mel1c and upregulated p-AKT, p-PKC, and p-ERK expression, thereby activating PI3K/AKT and PKC/ERK signaling and inducing B-lymphocyte proliferation. Overall, these findings suggested that melatonin modulates a combination of green and blue light-induced B-lymphocyte proliferation in chickens by reducing oxidative stress and activating the Mel1a/PI3K/AKT and Mel1c/PKC/ERK pathways.
人工照明可能会干扰鸟类的生物节律并破坏生理内稳态。我们之前的研究表明，660nm 红光会加剧氧化应激，但与单一单色光相比，绿光和蓝光（G→B）的组合可以提高鸡的抗体滴度。褪黑素作为一种抗氧化剂，对于协调外部光刺激和身体的细胞反应至关重要。本研究进一步阐明了褪黑素在单色光组合诱导鸡法氏囊 B 淋巴细胞增殖中的潜在作用。将 192 只小鸡暴露于单一单色光（红光（R）、绿光（G）、蓝光（B）或白光（W））或各种单色光组合（B→G、G→B 和 R→B）中，从 P0 到 P42。我们使用 qRT-PCR、MTT、western blot、免疫组织化学和 Elisa 来研究单色光组合对法氏囊 B 淋巴细胞及其细胞内信号通路的影响。随着血浆褪黑素水平和抗氧化酶能力的一致上调，我们观察到 G→B 组的器官指数、滤泡面积、淋巴细胞密度、B 淋巴细胞增殖、PCNA 阳性细胞和细胞周期蛋白 D1 表达增加，与其他光处理组相比。褪黑素与 Mel1a 和 Mel1c 结合，上调 p-AKT、p-PKC 和 p-ERK 的表达，从而激活 PI3K/AKT 和 PKC/ERK 信号通路，诱导 B 淋巴细胞增殖。总体而言，这些发现表明褪黑素通过减少氧化应激和激活 Mel1a/PI3K/AKT 和 Mel1c/PKC/ERK 通路来调节绿光和蓝光组合诱导的鸡 B 淋巴细胞增殖。
