Department of Pediatric Dentistry, Dental School, University of Marburg and University Medical Center Giessen and Marburg, Campus Giessen, Giessen, Germany.
Department of Operative Dentistry, Endodontics, and Pediatric Dentistry, Dental School, University of Marburg and University Medical Center Giessen and Marburg, Campus Marburg, Marburg, Germany.
Clin Exp Dent Res. 2021 Oct;7(5):763-771. doi: 10.1002/cre2.420. Epub 2021 Apr 7.
It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS).
This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application of self-etching primers/adhesives (SEPA).
131 third molars were assigned to ten groups. In Hel-P, Helioseal® was applied after phosphoric acid etching. In the other groups, SEPA were used (Dyr-AP: Adper™ Prompt™ L-Pop™, Dyract® Seal; Bea: BeautiSealant Primer and Paste; Hel-Exp: Experimental primer, Helioseal®; Hel-Cl: Clearfil™ SE Bond , Helioseal®). Specimens were stored in distilled water at 37°C (28 days), followed by 3500 thermocycles and staining with 5% methylene blue (M) or 5% silver nitrate (S). After methylene blue staining and sectioning, microleakage was assessed light microscopically. During silver nitrate staining, specimens were dissolved by 32% HCl and remaining PFS were evaluated under a SEM.
Tightness, percentage of penetrated area, and maximum dye penetration were best for Hel-P and Hel-Cl (p < 0.05).
Phosphoric acid etching of enamel and Clearfil™ SE Bond resulted in the best sealing quality. Methylene blue staining allowed the evaluation of more criteria (fissure shape, voids, sealant penetration depth) compared to silver nitrate.
对于获得窝沟封闭（PFS）最大密封性的最佳预处理方法，目前仍不完全清楚。
本研究调查了经磷酸酸蚀处理或应用自酸蚀底漆/黏合剂（SEPA）后进行 PFS 的微渗漏情况。
将 131 颗第三磨牙分为十组。在 Hel-P 组中，在磷酸酸蚀后应用 Helioseal®。在其他组中，使用 SEPA（Dyr-AP：Adper™ Prompt™ L-Pop™，Dyract® Seal；Bea：BeautiSealant Primer and Paste；Hel-Exp：实验底漆，Helioseal®；Hel-Cl：Clearfil™ SE Bond，Helioseal®）。将标本储存在 37°C 的蒸馏水中（28 天），随后进行 3500 次热循环，并使用 5%亚甲蓝（M）或 5%硝酸银（S）染色。亚甲蓝染色和切片后，用体视显微镜评估微渗漏。在硝酸银染色过程中，用 32%HCl 溶解标本，并用 SEM 评估剩余的 PFS。
对于 Hel-P 和 Hel-Cl，密封质量最好，包括紧密度、渗透面积百分比和最大染料渗透（p<0.05）。
磷酸酸蚀牙釉质和 Clearfil™ SE Bond 可获得最佳的密封质量。与硝酸银相比，亚甲蓝染色可评估更多的标准（窝沟形状、空隙、密封剂渗透深度）。
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