Sun Ya-Jun, Zhang Yan-Fang, Xu Hui-Min, Ma Yong-Tao, Li Chun, Nie Chun-Hong, Zhao Min
Department of Endocrinology, Kaifeng Children's Hospital, Kaifeng 475000, China.
PICU, Kaifeng Children's Hospital, Kaifeng 475000, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 Mar;52(2):229-234. doi: 10.12182/20210160507.
To investigate the effects of morin-regulated NLRP3/Caspase-1 pathway on experimental autoimmune thyroiditis in rats.
The rats were randomly assigned to 6 groups: control group, experimental autoimmune thyroiditis group (EAT), low-, medium- and high-dose morin groups (post-modeling gavage of 50, 100 and 200 mg/kg morin hydrate per day for 6 weeks) and tripterygium wilfordii polyglycosides group (LGT group, post-modeling gavage of 6.25 mg/kg tripterygium wilfordii polyglycosidesper day for 6 weeks). Except for the control group, the rat model of experimental autoimmune thyroiditis was established by subcutaneous injection of 0.1 mL incomplete Freund's adjuvant containing porcine thyroglobulin. The levels of serum thyroglobulin (TgAb), thyroid peroxidase antibody (TPOAb), triiodothyronine (T3) and tetraiodothyronine (T4) in serum were detected by radioimmunoassay. The mRNA levels of interleukin-17 ( -17), interleukin-4 ( -4) and interferon γ ( - ) were detected by reverse transcription-polymerase chain reaction. The levels of serum protein carbonyl content, 8-hydroxydeoxyguanosine (8-OHdG), and malondialdehyde (MDA) activity were checked with test kits. Expressions of NLRP3, apoptosis-related speck-like protein (ASC), and Caspase-1 were detected by Western blot.
Compared with the EAT group, serum levels of TPOAb, TgAb, T3, and T4 in low-, medium- and high-dose Morin groups and LGT group were reduced ( <0.01) and the mRNA levels of -17, and -4 were increased ( <0.01), the protein hydroxyl content, MDA activity, and 8-OHdG levels were reduced ( <0.01). The levels of NLRP3, ASC and Caspase-1 were reduced ( <0.01), the levels of 8-OHdG were significantly reduced ( <0.01), and the levels of NLRP3, ASC and Caspase-1 were significantly reduced ( <0.01). There were statistically significant differences between the data from the low-dose and the medium-dose Morin groups and the data of the LGT group ( <0.05), while data from the high-dose Morin group showed no significant difference compared with the data of the LGT group. Data from low-, medium- and high-dose Morin groups showed no statistically significant differences ( <0.05).
The findings suggest that Morin improved experimental autoimmune thyroiditis in rats through regulating NLRP3/Caspase-1 pathway.
探讨桑色素调节NLRP3/Caspase-1通路对大鼠实验性自身免疫性甲状腺炎的影响。
将大鼠随机分为6组：对照组、实验性自身免疫性甲状腺炎组（EAT）、低、中、高剂量桑色素组（造模后每天灌胃50、100和200 mg/kg桑色素水合物，共6周）和雷公藤多苷组（LGT组，造模后每天灌胃6.25 mg/kg雷公藤多苷，共6周）。除对照组外，通过皮下注射0.1 mL含猪甲状腺球蛋白的不完全弗氏佐剂建立大鼠实验性自身免疫性甲状腺炎模型。采用放射免疫分析法检测血清中甲状腺球蛋白（TgAb）、甲状腺过氧化物酶抗体（TPOAb）、三碘甲状腺原氨酸（T3）和四碘甲状腺原氨酸（T4）的水平。采用逆转录-聚合酶链反应检测白细胞介素-17（IL-17）、白细胞介素-4（IL-4）和干扰素γ（IFN-γ）的mRNA水平。用试剂盒检测血清蛋白羰基含量、8-羟基脱氧鸟苷（8-OHdG）和丙二醛（MDA）活性。采用蛋白质印迹法检测NLRP3、凋亡相关斑点样蛋白（ASC）和Caspase-1的表达。
与EAT组相比，低、中、高剂量桑色素组和LGT组血清TPOAb、TgAb、T3和T4水平降低（P<0.01），IL-17、IFN-γ和IL-4的mRNA水平升高（P<0.01），蛋白羟基含量、MDA活性和8-OHdG水平降低（P<0.01）。NLRP3、ASC和Caspase-1水平降低（P<0.01），8-OHdG水平显著降低（P<0.01），NLRP3、ASC和Caspase-1水平显著降低（P<0.01）。低剂量和中剂量桑色素组的数据与LGT组的数据之间存在统计学差异（P<0.05），而高剂量桑色素组的数据与LGT组的数据相比无显著差异。低、中、高剂量桑色素组的数据无统计学差异（P>0.05）。
研究结果表明，桑色素通过调节NLRP3/Caspase-1通路改善大鼠实验性自身免疫性甲状腺炎。
