State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, National Demonstration Center for Experimental, Biomedical Engineering Education, Southeast University, Nanjing 210096, China.
Institute for Brain and Intelligence, Southeast University, Nanjing 210096, China.
Langmuir. 2021 Apr 20;37(15):4441-4448. doi: 10.1021/acs.langmuir.0c03551. Epub 2021 Apr 8.
Antifouling materials have many important applications in biomedical devices and marine coating. Oligo(ethylene glycol) (OEG) or poly(ethylene glycol) (PEG) exhibit promising antifouling properties and are widely used in biomedical engineering. Chiral selection is an important phenomenon in biological processes. Because of the influence of steric hindrance, the modification of chiral molecules with different chirality at interfaces will affect the intermolecular interaction at the interfaces and lead to different structures of interfacial molecules. The difference of surface structures such as surface hydration structure would impact the adsorption of biomolecules on the surface, thus causing different varieties of cell adhesion and cell growth. In this study, the influence on surface hydration and surface cell adhesion of OEG self-assembled monolayers (SAMs) modified with cysteine showing different chirality are explored. The water structure at the interfaces of OEG/water in different conditions was probed with sum frequency generation vibrational spectroscopy (SFG-VS). The results show that the interfacial water structure can change significantly with either d-cysteine or l-cysteine modification on OEG. Water molecules are more ordered at the OEG/water interface under the d-cysteine modification on OEG SAMs, which improves the protein adsorption resistance of the surface. In contrast, l-cysteine modification would make the water less ordered at the OEG/protein solution interface and enhance the protein adsorption. Additionally, optical micrographs indicate that l-cysteine can significantly promote the OEG SAMs cell adhesion and growth, while d-cysteine exhibits an inhibitory effect, which is consistent with the results of SFG-VS experiments.
抗污材料在生物医学设备和海洋涂料中有许多重要的应用。聚乙二醇（PEG）或聚乙二醇（OEG）具有良好的抗污性能，广泛应用于生物医学工程中。手性选择是生物过程中的一个重要现象。由于空间位阻的影响，不同手性的手性分子在界面上的修饰会影响界面上的分子间相互作用，导致界面分子的不同结构。表面结构的差异，如表面水化结构，会影响生物分子在表面的吸附，从而导致不同种类的细胞黏附和细胞生长。在这项研究中，探讨了具有不同手性的半胱氨酸修饰的聚乙二醇自组装单分子层（SAM）对表面水化和表面细胞黏附的影响。利用和频产生振动光谱（SFG-VS）探测不同条件下 OEG/水界面的水结构。结果表明，OEG 上的 d-半胱氨酸或 l-半胱氨酸修饰可以显著改变界面水结构。在 OEG SAMs 上 d-半胱氨酸修饰下，界面水分子的有序性增加，从而提高了表面的蛋白质抗吸附能力。相比之下，l-半胱氨酸修饰会降低 OEG/蛋白质溶液界面处的水有序性，增强蛋白质吸附。此外，光学显微镜图像表明，l-半胱氨酸可以显著促进 OEG SAMs 的细胞黏附和生长，而 d-半胱氨酸则表现出抑制作用，这与 SFG-VS 实验结果一致。
