Medical Science Division, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1194, Japan.
Research and Development Department, Ichimaru Pharcos Co., Ltd, 318-1 Asagi, Motosu City, Gifu, 501-0475, Japan.
J Nat Med. 2021 Jun;75(3):623-632. doi: 10.1007/s11418-021-01509-x. Epub 2021 Apr 8.
The anti-inflammatory effects of a 50% aqueous extract of Rosa roxburghii fruit (RRFE) and two ellagitannins (strictinin and casuarictin) isolated from the RRFE were evaluated in a cell model of skin inflammation induced by self-RNA released from epidermal cells damaged by UV ray (UVR) irradiation. The RRFE inhibited interleukin-8 (IL-8) mRNA expression in normal human epidermal keratinocytes (NHEKs) stimulated with polyinosinic:polycytidylic acid (poly(I:C)), a ligand of toll-like receptor-3 (TLR-3). The plant-derived anti-inflammatory agents, dipotassium glycyrrhizinate (GK2) and allantoin, had no influence on the IL-8 expression. The purified compounds, strictinin and casuarictin, inhibited the IL-8 mRNA expression and IL-8 release induced in NHEKs by poly(I:C). These ellagitannins were thus found to be responsible for the biological activity exhibited by the RRFE. This study demonstrates that RRFE and isolated RRFE compounds show promise as ingredients for products formulated to improve skin disorders induced by UVR irradiation.
采用紫外线（UVR）辐射损伤表皮细胞释放的自身 RNA 诱导的皮肤炎症细胞模型，评价蔷薇果 50%水提物（RRFE）及其从 RRFE 中分离得到的两种鞣花单宁（鞣花丹宁和原诃子酸）的抗炎作用。RRFE 抑制多聚肌苷酸：多聚胞苷酸（poly（I：C））刺激的正常人表皮角质形成细胞（NHEK）白细胞介素-8（IL-8）mRNA 表达，poly（I：C）是 Toll 样受体-3（TLR-3）的配体。植物来源的抗炎剂甘草酸二钾（GK2）和尿囊素对 IL-8 表达没有影响。纯化的化合物鞣花丹宁和原诃子酸抑制 poly（I：C）诱导的 NHEK 中 IL-8 mRNA 表达和 IL-8 释放。因此，这些鞣花单宁被认为是 RRFE 表现出生物活性的原因。本研究表明，RRFE 和分离的 RRFE 化合物有望成为产品的成分，用于改善 UVR 照射引起的皮肤疾病。
