Department of Plant Biology, Rutgers University, New Brunswick, New Jersey 08901, United States.
Center for Natural Product Technologies, Pharmacognosy Institute, and Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Illinois 60612, United States.
J Nat Prod. 2021 Apr 23;84(4):1078-1086. doi: 10.1021/acs.jnatprod.0c01149. Epub 2021 Apr 8.
Two new diprenylated coumaric acid isomers ( and ) and two known congeners, capillartemisin A () and B (), were isolated from as bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant's 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed with extract.
从 中分离到两种新的二苯乙烯基咖啡酸异构体（ 和 ）和两种已知的同系物，毛兰素 A () 和 B ()，作为生物活性标记物，使用基于生物活性的 HPLC 分级分离。通过光谱方法，包括 1D 和 2D NMR 方法和 LC-MS，确定了它们的结构，并通过 1D H 纯位移 qNMR 光谱分析评估其纯度。通过油红 O 染色测量脂质的积累增加以及几种脂肪细胞标记基因（包括脂肪细胞中的脂联素）的表达增加来评估化合物的生物活性，与未处理的阴性对照相比，3T3-L1 脂肪细胞中的表达增加。与植物的 80%EtOH 提取物相比，这些纯化的化合物在 3T3-L1 脂肪细胞中对 TNFα 诱导的脂肪分解的抑制作用虽然显著，但仍然较弱。这表明，对于用 提取物观察到的对脂肪细胞的多种代谢有益的影响，还有其他生物活性物质负责。
