从中分离得到的倍半萜对 3T3-L1 前体脂肪细胞的成脂分化的抑制作用。

Inhibitory Effects of Sesquiterpenoids Isolated from on Adipogenic Differentiation of 3T3-L1 Preadipocytes.

机构信息

Incheon Regional Office, National Fishery Products Quality Management Service Incheon, Incheon 22346, Republic of Korea.

Nutritional Education, Graduate School of Education, Silla University, Busan 46958, Republic of Korea.

出版信息

Int J Mol Sci. 2023 Dec 22;25(1):200. doi: 10.3390/ijms25010200.

DOI:10.3390/ijms25010200

PMID:38203371

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10779302/

Abstract

Obesity and related complications are significant health issues in modern society, largely attributed to a sedentary lifestyle and a carbohydrate-rich diet. Since anti-obesity drugs often come with severe side effects, preventative measures are being sought globally, including dietary changes and functional foods that can counteract weight gain. In this context, plant-based metabolites are extensively studied for their advantageous biological effects against obesity. Several plants within the genus have been reported to possess anti-adipogenic properties, preventing adipocytes from maturing and accumulating lipids. The present study investigated the anti-adipogenic potential of two sesquiterpenoids, reynosin and santamarine, isolated from in adipose-induced 3T3-L1 preadipocytes. Differentiating 3T3-L1 adipocytes treated with these isolated compounds displayed fewer adipogenic characteristics compared to untreated mature adipocytes. The results indicated that cells treated with reynosin and santamarine accumulated 55.0% and 52.5% fewer intracellular lipids compared to untreated control adipocytes, respectively. Additionally, the mRNA expression of the key adipogenic marker, transcription factor PPARγ, was suppressed by 87.2% and 91.7% following 60 μM reynosin and santamarine treatment, respectively, in differentiated adipocytes. Protein expression was also suppressed in a similar manner, at 92.7% and 82.5% by 60 μM reynosin and santamarine treatment, respectively. Likewise, SERBP1c and C/EBPα were also downregulated at both gene and protein levels in adipocytes treated with samples during differentiation. Further analysis suggested that the anti-adipogenic effect of the compounds might be a result of AMPK activation and the subsequent suppression of MAPK phosphorylation. Overall, the present study suggested that sesquiterpenoids, reynosin, and santamarine were two potential bioactive compounds with anti-adipogenic properties. Further research is needed to explore other bioactive agents within and elucidate the in vivo action mechanisms of reynosin and santamarine.

摘要

肥胖及相关并发症是现代社会的重大健康问题，主要归因于久坐的生活方式和富含碳水化合物的饮食。由于抗肥胖药物通常伴随着严重的副作用，因此全球都在寻求预防措施，包括饮食改变和具有抗体重增加作用的功能性食品。在这种情况下，植物代谢物因其对肥胖的有利生物学作用而被广泛研究。已经有报道称，属于该属的几种植物具有抗脂肪生成特性，可阻止脂肪细胞成熟和积累脂质。本研究调查了从分离得到的两种倍半萜类化合物 Reynosin 和 Santamarine 对脂肪诱导的 3T3-L1 前体脂肪细胞的抗脂肪生成潜力。与未处理的成熟脂肪细胞相比，用这些分离化合物处理的分化 3T3-L1 脂肪细胞显示出较少的脂肪生成特征。结果表明，与未处理的对照脂肪细胞相比，用 Reynosin 和 Santamarine 处理的细胞分别积累了 55.0%和 52.5%更少的细胞内脂质。此外，在分化的脂肪细胞中，60 μM Reynosin 和 Santamarine 处理分别抑制了关键脂肪生成标记物转录因子 PPARγ 的 mRNA 表达 87.2%和 91.7%。蛋白质表达也以相似的方式受到抑制，分别为 60 μM Reynosin 和 Santamarine 处理时的 92.7%和 82.5%。同样，在分化过程中用样品处理脂肪细胞时，SERBP1c 和 C/EBPα 的基因和蛋白质水平也下调。进一步分析表明，化合物的抗脂肪生成作用可能是 AMPK 激活的结果，随后抑制了 MAPK 磷酸化。总的来说，本研究表明倍半萜类化合物 Reynosin 和 Santamarine 是两种具有抗脂肪生成特性的潜在生物活性化合物。需要进一步研究来探索该属内的其他生物活性物质，并阐明 Reynosin 和 Santamarine 的体内作用机制。