Shen Xiaoyun, Huo Bin, Li Yuanfeng, Song Chunjie, Wu Ting, He Jian
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China; State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, Xinjiang, China; World Bank Poverty Alleviation Project Office in Guizhou, Southwest China, Guiyang 550004, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
J Proteomics. 2021 Jun 15;241:104218. doi: 10.1016/j.jprot.2021.104218. Epub 2021 Apr 5.
Selenium (Se) is an essential mineral nutrient for animals. Se deprivation can lead to many disorders and even death. This study investigated the response of Przewalski's gazelle (P. przewalskii) to Se-deprived environment. We found that Se deprivation in soil and forage not only influenced the mineral contents of the blood and hair in P. przewalskii, but also severely disrupted their blood parameters. We identified significant changes in the abundance of 146 proteins and 25 metabolites (P < 0.05) in serum, including the selenoproteins L8IG93 (glutathione peroxidase) and F4YD09 (Cu/Zn superoxide dismutase). Furthermore, the major known proteins and metabolites associated with the Se stress response in P. przewalskii were Cu/Zn superoxide dismutase, the vitamin K-dependent protein C, the C4b-binding protein alpha chain, complement component C7, lipase linoleic acid, peptidase D, thymidine, pseudo-uridine, L-phenylalanine, L-glutamine, PGA1, and 15-deoxy-delta-12,14-PGJ2. The main signaling pathways involved included complement and coagulation cascades, metabolic pathways, and stress granule formation. Our results indicate that the intake of Se-deficient forage elicited an oxidative stress response in P. przewalskii. These findings provide insights into the response mechanisms of this threatened gazelle to Se stress, and enable the development of conservation strategies to protect populations on the Qinghai-Tibetan Plateau. SIGNIFICANCE: This study is the first to point out the presence of oxidative stress in P. przewalskii in selenium-deficient areas through proteomics and metabolomics studies. These findings should prove helpful for conservation efforts aimed at P. przewalskii populations and maintenance of the integrity of their ecological environment.
硒（Se）是动物必需的矿物质营养素。缺硒会导致多种疾病甚至死亡。本研究调查了普氏原羚对缺硒环境的反应。我们发现土壤和饲料中缺硒不仅影响了普氏原羚血液和毛发中的矿物质含量，还严重扰乱了它们的血液参数。我们鉴定出血清中146种蛋白质和25种代谢物的丰度有显著变化（P < 0.05），包括硒蛋白L8IG93（谷胱甘肽过氧化物酶）和F4YD09（铜/锌超氧化物歧化酶）。此外，与普氏原羚硒应激反应相关的主要已知蛋白质和代谢物是铜/锌超氧化物歧化酶、维生素K依赖蛋白C、C4b结合蛋白α链、补体成分C7、亚油酸脂肪酶、肽酶D、胸苷、假尿苷、L-苯丙氨酸、L-谷氨酰胺、PGA1和15-脱氧-δ-12,14-PGJ2。涉及的主要信号通路包括补体和凝血级联、代谢途径以及应激颗粒形成。我们的结果表明，摄入缺硒饲料会引发普氏原羚的氧化应激反应。这些发现为这种濒危羚羊对硒应激的反应机制提供了见解，并有助于制定保护青藏高原种群的保护策略。意义：本研究首次通过蛋白质组学和代谢组学研究指出缺硒地区普氏原羚存在氧化应激。这些发现应有助于针对普氏原羚种群的保护工作及其生态环境完整性的维护。
