Huo Bin, He Jian, Shen Xiaoyun
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
World Bank Poverty Alleviation Project Office in Guizhou, Southwest China, Guiyang, 550004, China.
Biol Trace Elem Res. 2020 Nov;198(1):149-156. doi: 10.1007/s12011-020-02070-6. Epub 2020 Feb 10.
Przewalski's gazelle (Procapra przewalskii) is an endangered ungulate in the Qinghai-Tibet Plateau of China. This study aimed to determine the influence of selenium (Se) deprivation in the natural habitat on the immune index and antioxidant capacity of P. przewalskii. Samples of soil and forage were collected from affected and healthy areas, and animal tissues were collected from affected and healthy P. przewalskii. The samples were used for measuring mineral content and for hematological and biochemical analyses. The results showed that Se concentrations were significantly lower in the soil and mixed forage samples from the affected area than in those from the healthy area. The Se concentrations were significantly lower in blood and hair samples from affected P. przewalskii than in those from healthy P. przewalskii. Meanwhile, hemoglobin, packed cell volume, and platelet count of affected P. przewalskii were significantly lower than those of healthy P. przewalskii. The serum level of glutathione peroxidase and total antioxidant capacity were significantly lower and the serum levels of malondialdehyde, total nitric oxide synthase, and lipid peroxide were significantly higher in affected P. przewalskii. The serum levels of interleukin (IL)-1β, IL-2, tumor necrosis factor-alpha, immunoglobulin A (IgA), and IgG significantly decreased and the serum levels of IL-6 and IgM significantly reduced in affected P. przewalskii compared with healthy P. przewalskii. Therefore, the findings indicated that Se deprivation in soil and forage caused oxidative stress damage and posed a serious threat to the immune function of P. przewalskii.
普氏原羚（Procapra przewalskii）是中国青藏高原的一种濒危有蹄类动物。本研究旨在确定自然栖息地中硒（Se）缺乏对普氏原羚免疫指标和抗氧化能力的影响。从受影响地区和健康地区采集土壤和草料样本，并从受影响和健康的普氏原羚身上采集动物组织样本。这些样本用于测量矿物质含量以及进行血液学和生化分析。结果表明，受影响地区土壤和混合草料样本中的硒浓度显著低于健康地区。受影响的普氏原羚血液和毛发样本中的硒浓度显著低于健康普氏原羚。同时，受影响的普氏原羚的血红蛋白、红细胞压积和血小板计数显著低于健康普氏原羚。受影响的普氏原羚血清中的谷胱甘肽过氧化物酶水平和总抗氧化能力显著降低，而丙二醛、总一氧化氮合酶和脂质过氧化物的血清水平显著升高。与健康普氏原羚相比，受影响的普氏原羚血清中的白细胞介素（IL）-1β、IL-2、肿瘤坏死因子-α、免疫球蛋白A（IgA）和IgG水平显著降低，IL-6和IgM的血清水平显著下降。因此，研究结果表明，土壤和草料中的硒缺乏会导致氧化应激损伤，并对普氏原羚的免疫功能构成严重威胁。
