Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China.
Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China.
Chemosphere. 2021 Sep;278:130428. doi: 10.1016/j.chemosphere.2021.130428. Epub 2021 Apr 1.
The integration of constructed wetland-microbial fuel cell (CW-MFC) and anaerobic granular sludge (AGS) is an important way to promote its ammonification efficiency and decrease the land use scale. This study explored the integration of CW-MFC and AGS for nitrogen removal via the intensified ammonification-nitrification-denitrification processes with initial NH-N, NO-N, Org-N and total nitrogen (TN) concentrations of 10.5, 13.8, 21.4, and 45.7 mg L in wastewater. Two reactors with AGS inoculated with a separated area (R1) and directly inoculated into gravel substrate (R2) were designed, respectively. Results showed that chemical oxygen demand (COD) removal efficiency could reach 85% in R1 and 81% in R2, and the conversion of Org-N to NH-N and NO-N to gaseous nitrogen were 80% and 90%, respectively. Although the conversion efficiency of NH-N to NO-N/NO-N via nitrification process was only 18%, it could reach 45%, 94%, and 98% with the aeration rates of 50-, 100-, and 200-mL min. According to microstructural property and microbial community analyses, the separation gravel substrate and AGS areas in R1 availed for stable particle size of AGS, archaeal diversity, and metabolic activity even with a 1.5 times daily wastewater treatment capacity than that of R2. Overall, although the intensified ammonification-nitrification-denitrification processes for nitrogen removal could be achieved with supplementary aeration, further investigation is still needed to explore other substrate materials and high CW-MFC/AGS volume ratio for intensified nitrification process in CW-MFC associated with AGS.
将人工湿地-微生物燃料电池（CW-MFC）与厌氧颗粒污泥（AGS）集成是提高氨化效率和降低土地利用规模的重要途径。本研究通过强化氨化-硝化-反硝化过程，利用初始 NH-N、NO-N、Org-N 和总氮（TN）浓度分别为 10.5、13.8、21.4 和 45.7mg/L 的废水，探索了 CW-MFC 与 AGS 集成用于脱氮。设计了两个分别用分离区接种 AGS 的反应器（R1）和直接接种砾石基质的反应器（R2）。结果表明，R1 的 COD 去除效率可达 85%，R2 可达 81%，Org-N 向 NH-N 和 NO-N 的转化率分别为 80%和 90%。尽管硝化过程中 NH-N 向 NO-N/NO-N 的转化率仅为 18%，但在曝气率为 50、100 和 200mL/min 时，可分别达到 45%、94%和 98%。根据微观结构特性和微生物群落分析，R1 中的分离砾石基质和 AGS 区域有利于 AGS 稳定的颗粒大小、古菌多样性和代谢活性，即使处理能力是 R2 的 1.5 倍。总的来说，尽管通过补充曝气可以实现强化氨化-硝化-反硝化脱氮过程，但仍需要进一步研究，以探索其他基质材料和高 CW-MFC/AGS 体积比，以促进 CW-MFC 与 AGS 相关的硝化过程。
