Guangdong Technology Research Center for Marine Algal Bioengineering, Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China; Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen, 518055, China.
Guangdong Technology Research Center for Marine Algal Bioengineering, Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China.
Chemosphere. 2023 Apr;321:138157. doi: 10.1016/j.chemosphere.2023.138157. Epub 2023 Feb 14.
We undertook a lab-scale evaluation of a novel autotrophic denitrification and nitrification integrated constructed wetland (ADNI-CW) for improved carbon (C), nitrogen (N), and sulfur (S) cycling to treat mariculture wastewater. The process involved an up-flow autotrophic denitrification constructed wetland unit (AD-CW) for sulfate reduction and autotrophic denitrification, and an autotrophic nitrification constructed wetland unit (AN-CW) for nitrification. The 400-day experiment investigated the performance of the AD-CW, AN-CW, and entire ADNI-CW processes under various hydraulic retention times (HRTs), nitrate concentrations, dissolved oxygen levels, and recirculation ratios. Under various HRTs, the AN-CW achieved a nitrification performance exceeding 92%. Correlation analysis of the chemical oxygen demand (COD) revealed that, on average, approximately 96% of COD was removed by sulfate reduction. Under different HRTs, increases in influent NO-N concentrations caused the amount of sulfide to gradually decrease from sufficient to deficient, and the autotrophic denitrification rate also decreased from 62.18 to 40.93%. In addition, when the NO-N load rate was above 21.53 g N/m·d, the transformation of organic N by mangrove roots may have increased NO-N in the top effluent of the AD-CW. The coupling of N and S metabolic processes mediated by various functional microorganisms (Proteobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, and unclassified_d__Bacteria) enhanced N removal. We intensively explored the effects of changing inputs as culture species developed on the physical, chemical, and microbial changes of CW to ensure a consistent and effective management of C, N, and S. This study lays the foundation for green and sustainable mariculture development.
我们进行了一项实验室规模的新型自养反硝化和硝化一体化人工湿地（ADNI-CW）评估，以改善碳（C）、氮（N）和硫（S）循环，处理海水养殖废水。该工艺涉及一个上流式自养反硝化人工湿地单元（AD-CW）用于硫酸盐还原和自养反硝化，以及一个自养硝化人工湿地单元（AN-CW）用于硝化。在 400 天的实验中，研究了不同水力停留时间（HRT）、硝酸盐浓度、溶解氧水平和回流比下 AD-CW、AN-CW 和整个 ADNI-CW 工艺的性能。在不同的 HRT 下，AN-CW 的硝化性能超过 92%。对化学需氧量（COD）的相关分析表明，硫酸盐还原平均去除了约 96%的 COD。在不同的 HRT 下，进水 NO-N 浓度的增加导致硫化物的量逐渐从充足变为不足，自养反硝化速率也从 62.18 下降到 40.93%。此外，当 NO-N 负荷率高于 21.53 g N/m·d 时，红树林根系对有机 N 的转化可能会增加 AD-CW 顶部出水中的 NO-N。各种功能微生物（变形菌门、绿弯菌门、放线菌门、拟杆菌门和未分类的 d__Bacteria）介导的 N 和 S 代谢过程的耦合增强了 N 的去除。我们深入探讨了随着养殖物种的发展，改变输入对 CW 物理、化学和微生物变化的影响，以确保 C、N 和 S 的持续有效管理。这项研究为绿色可持续海水养殖发展奠定了基础。
