School of Engineering, University of Guelph, Ontario N1G 2W1, Canada.
School of Engineering, University of Guelph, Ontario N1G 2W1, Canada; College of Life Sciences, Fujian Normal University, Fuzhou 350117, China.
Bioresour Technol. 2021 Jul;331:125029. doi: 10.1016/j.biortech.2021.125029. Epub 2021 Mar 27.
Numerous studies have revealed the effect of temperature and hydraulic retention time (HRT) on microbiota in sludge biological hydrolysis (BH). However, few scholars have explored the combined effect of these two critical BH parameters. This study explored the BH performance and community structures over 12 combined temperatures-HRT conditions for temperatures from 35 °C to 55 °C and HRTs from 1.5 days to 6.0 days. Results showed that the 12 combined conditions formed only six distinct community structures with each of them relating to a distinctive range of volatile suspended solid reduction rates. The nonmetric multidimensional scaling and species-species association analysis on the DNA sequencing data revealed that the community structure was greatly driven by the microbial interactions (e.g., heterogeneous commensalism and competition) under the effect of temperature and HRT. This study established the linkages among the combined BH temperature-HRT conditions, microbial interaction, microbial community, and BH performance.
许多研究揭示了温度和水力停留时间（HRT）对污泥生物水解（BH）中微生物群的影响。然而，很少有学者探索这两个关键 BH 参数的综合影响。本研究在 35°C 至 55°C 的温度和 1.5 天至 6.0 天的 HRT 条件下，探索了 12 种组合温度-HRT 条件下的 BH 性能和群落结构。结果表明，在 12 种组合条件下仅形成了 6 种独特的群落结构，每种结构都与挥发性悬浮固体减少率的独特范围相关。DNA 测序数据的非度量多维标度和种间关联分析表明，在温度和 HRT 的影响下，群落结构主要受微生物相互作用（例如，异质共生和竞争）的驱动。本研究建立了组合 BH 温度-HRT 条件、微生物相互作用、微生物群落和 BH 性能之间的联系。
