School of Engineering, University of Guelph, Ontario N1G 2W1, Canada.
School of Engineering, University of Guelph, Ontario N1G 2W1, Canada.
Bioresour Technol. 2017 Dec;245(Pt A):502-510. doi: 10.1016/j.biortech.2017.08.143. Epub 2017 Aug 24.
This study investigated the biological hydrolysis performance at 35°C (BH35), 42°C (BH42), and 55°C (BH55) and the effect of temperatures on microbial communities of the hydrolyzed sludge. The results showed that the suspended solid reduction, volatile fatty acids (VFA) production, and biogas production increased with the BH temperatures. VFAs produced in the sludge BH included acetic acid, propionic acid, isobutyric acid, butyric acid, and isovaleric acid with the fractions of acetic acid increased with BH temperatures. The Illumina MiSeq sequencing analysis showed that the microbial taxonomic structures of the BH systems varied with BH temperatures. It was found that Acidaminobacter at 35°C, Proteiniphilum and Lutispor at 42°C, and Gelria at 55°C were the main protein fermenting bacteria genera, while the carbohydrate fermenting bacteria might belong to the genera of Macellibacteroides and Paludibacter at 35°C, Fronticella at 42°C, and Tepidimicrobium at 55°C.
本研究考察了 35°C（BH35）、42°C（BH42）和 55°C（BH55）下的生物水解性能，以及温度对水解污泥中微生物群落的影响。结果表明，悬浮固体减少、挥发性脂肪酸（VFA）生成和沼气生成随着 BH 温度的升高而增加。污泥 BH 中产生的 VFAs 包括乙酸、丙酸、异丁酸、丁酸和异戊酸，随着 BH 温度的升高，乙酸的比例增加。Illumina MiSeq 测序分析表明，BH 系统的微生物分类结构随 BH 温度而变化。结果发现，35°C 时主要的蛋白发酵菌属为 Acidaminobacter，42°C 时为 Proteiniphilum 和 Lutispor，55°C 时为 Gelria，而碳水化合物发酵菌可能属于 Macellibacteroides 和 Paludibacter（35°C）、Fronticella（42°C）和 Tepidimicrobium（55°C）。
