果蝇胚胎中形态发生素的梯度分布。（注：bicoid protein一般译为形态发生素，这里根据语境意译了。原译文不太准确，正确的应该是和果蝇胚胎发育相关的某种蛋白梯度，这里意译为形态发生素梯度分布更符合语境）正确译文：果蝇胚胎中形态发生素bicoid的梯度分布。（如果bicoid有特定的专业中文译名，可替换“形态发生素bicoid”）

A gradient of bicoid protein in Drosophila embryos.

作者信息

Driever W, Nüsslein-Volhard C

机构信息

Max-Planck-Institut für Entwicklungsbiologie, Abteilung III Genetik, Tübingen, Federal Republic of Germany.

出版信息

Cell. 1988 Jul 1;54(1):83-93. doi: 10.1016/0092-8674(88)90182-1.

DOI:10.1016/0092-8674(88)90182-1

PMID:3383244

Abstract

The maternal gene bicoid (bcd) organizes anterior development in Drosophila. Its mRNA is localized at the anterior tip of the oocyte and early embryo. Antibodies raised against bcd fusion proteins recognize a 55-57 kd doublet band in Western blots of extracts of 0-4 hr old embryos. This protein is absent or reduced in embryonic extracts of nine of the 11 bcd alleles. The protein is concentrated in the nuclei of cleavage stage embryos. It cannot be detected in oocytes, indicating temporal control of bcd mRNA translation. The bcd protein is distributed in an exponential concentration gradient with a maximum at the anterior tip, reaching background levels in the posterior third of the embryo. The gradient is probably generated by diffusion from the local mRNA source and dispersed degradation.

摘要

母源基因双胸（bcd）在果蝇中调控前部发育。其mRNA定位于卵母细胞和早期胚胎的前端。针对bcd融合蛋白产生的抗体在0 - 4小时龄胚胎提取物的蛋白质免疫印迹中识别出一条55 - 57 kd的双峰带。在11个bcd等位基因中的9个的胚胎提取物中，这种蛋白质缺失或减少。该蛋白质集中在卵裂期胚胎的细胞核中。在卵母细胞中无法检测到，这表明bcd mRNA的翻译存在时间控制。bcd蛋白以指数浓度梯度分布，在前部顶端达到最大值，在胚胎后部三分之一处达到背景水平。这种梯度可能是由从局部mRNA来源的扩散和分散降解产生的。