Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong, China.
Department of Biomedical Engineering, Shandong Provincial Hospital affiliated to Shandong First Medical University, Shandong, China.
Pak J Pharm Sci. 2020 Sep;33(5(Special)):2445-2448.
The aim of the study was to establish reference intervals (RIs) for glomerular filtration function markers among pregnant women of Shandong Province, east China. From Janunary 2017 to December 2018, we retrospectively analyzed serum samples from 360 pregnant women and a control cohort of 60-non-pregnant women. The glomerular filtration function markers included Cystatin C (CysC), Creatinine (Cr) and Estimated Glomerular Filtration Rate (eGFR). BeckmanAU5800 detection system was used to determine the serological level of CysC by immunonephelometry method and Cr by enzyme method, eGFR was calculated according to age, gender and Cr results. We calculated the RIs according to the guidelines in C28-A3 published by the Clinical and Laboratory Standards Institute (CLSI). The calculated RIs for serum CysC were (0.40-0.67) mg/L, (0.5-0.85) mg/L, (0.77-1.49) mg/L in 1st, 2nd, and 3rd trimester respectively. Cr were (37.26-57.47) μmol/L, (33.70-54.82) μmol/L, (33.66-62.69) μmol/L in each cohort. eGFR based on Cr were (115.24-140.05) ml/min per 1.73m, (117.42-141.88) ml/min per 1.73m2, (109.00-146.00) ml/min per 1.73m2. The results show the necessity to establish special RIs for glomerular filtration function markers during pregnancy, even in each trimester. CysC levels increase obviously, so we also should cautiously treat it in the three trimesters.
本研究旨在建立中国东部山东省孕妇肾小球滤过功能标志物的参考区间（RI）。我们回顾性分析了 2017 年 1 月至 2018 年 12 月期间 360 名孕妇和 60 名非孕妇的血清样本。肾小球滤过功能标志物包括胱抑素 C（CysC）、肌酐（Cr）和估算肾小球滤过率（eGFR）。采用贝克曼 AU5800 检测系统，通过免疫比浊法测定 CysC 的血清水平，酶法测定 Cr，根据年龄、性别和 Cr 结果计算 eGFR。我们根据临床和实验室标准协会（CLSI）发布的 C28-A3 指南计算 RI。血清 CysC 的计算 RI 分别为 1 期、2 期和 3 期的（0.40-0.67）mg/L、（0.5-0.85）mg/L 和（0.77-1.49）mg/L。Cr 分别为各期的（37.26-57.47）μmol/L、（33.70-54.82）μmol/L 和（33.66-62.69）μmol/L。基于 Cr 的 eGFR 分别为（115.24-140.05）ml/min/1.73m、（117.42-141.88）ml/min/1.73m2 和（109.00-146.00）ml/min/1.73m2。结果表明，即使在每个孕期，建立孕妇肾小球滤过功能标志物特殊 RI 也是必要的。CysC 水平明显升高，因此我们在三个孕期也应谨慎对待。
