Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Department of Food & Biomedical Engineering, NED University of Engineering and Technology, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Sep;33(5(Supplementary)):2293-2300.
The current study was designed to determine the outcome of banana fruit pulp (BFP) on repeated noise stress exposure (NSE)-induced behavioral deficits and oxidative stress in male mice. BFP (600mg/kg b.w) was administered orally once daily for 2 weeks prior exposure to noise stress. Mice were exposed to NS for 4 h after administration of BFP for 2 weeks. Control mice were administered drinking water and similar treatment as given to test animals. At the end of the treatment behavioral changes were monitored. Animals were sacrificed following behavioral assessment and the brain and plasma samples were collected for biochemical analysis. Repeated NS-induced behavioral deficits (anxiety and depression), impaired learning and memory and produced oxidative stress. Administration of BFP inhibited NS-induced behavioral deficits (anxiolytic and antidepressant effects) and improved cognitive abilities. Brain lipid per oxidation was also decreased with concomitant increase of antioxidant enzyme activities. Repeated noise stress increased plasma corticosterone levels. A significant decrease of plasma corticosterone was observed on unstressed BFP treated animals while this decrease was comparable in stressed + BFP animals. Decreased levels of acetylcholinesterase in BPF+NS treated animals indicated increased cholinergic function which improves learning and memory. Repeated oral administration of BFP induced cognitive improving ability, anti-stress effect and potentiated antioxidant defence mechanism in both control and NS mice. Thus, it is suggested that dietary supplementation of BFP has a curative effect against NS-induced psychiatric and cognitive related disorders which merits deliberation and additional appraisal.
本研究旨在探讨香蕉果肉（BFP）对反复噪声应激暴露（NSE）引起的雄性小鼠行为缺陷和氧化应激的影响。BFP（600mg/kg b.w）在暴露于噪声应激前每天口服给药一次，共 2 周。在 BFP 给药 2 周后，小鼠接受 4 小时的 NS。对照组小鼠给予饮用水和与试验动物相同的处理。在治疗结束时监测行为变化。在行为评估后处死动物，并采集脑和血浆样本进行生化分析。反复的 NS 诱导的行为缺陷（焦虑和抑郁）、学习和记忆受损以及氧化应激。BFP 的给药抑制了 NS 诱导的行为缺陷（抗焦虑和抗抑郁作用），并改善了认知能力。脑脂质过氧化也减少，同时抗氧化酶活性增加。反复的噪声应激增加了血浆皮质酮水平。在未受应激的 BFP 处理动物中观察到血浆皮质酮水平显著降低，而在应激+BFP 动物中这种降低是相当的。在 BPF+NS 处理动物中乙酰胆碱酯酶水平降低表明胆碱能功能增强，从而改善学习和记忆。BFP 的重复口服给药在对照和 NS 小鼠中均诱导认知改善能力、抗应激作用和增强抗氧化防御机制。因此，建议膳食补充 BFP 对 NS 诱导的精神和认知相关障碍具有治疗作用，值得进一步研究和评估。
