异硫氰酸荧光素标记凝集素在糖类免疫组织学显示中的应用。III. 蓖麻凝集素和麦胚凝集素的研究

The use of fluorescein isothiocyanate labelled lectins for immuno-histological demonstration of saccharides. III. Studies by use of Ricinus communis lectin and wheat germ agglutinin.

作者信息

Thoss K, Roth J

出版信息

Exp Pathol (Jena). 1977 Sep-Oct;14(3-4):215-9. doi: 10.1016/s0014-4908(77)80069-0.

Abstract

Cryostat sections of heart, skeletal muscle, spleen, liver, cartilage, brain, nerve, tongue, esophagus and salivary gland from man, rabbit, rat and guinea pig were stained with fluorescein isothiocyanate labelled Ricinus communis lectin and wheat germ agglutinin. The stained sections were examined by fluorescence microscopy. The general pattern of fluorescence is very similar to that obtained with labelled Lens culinaris lectin and Concanavalin A. Particularly the lectins stain connective tissue structures of the organs. The staining intensity in these structures was weaker by wheat germ agglutinin as compared to the other lectins.

摘要

用人、兔、大鼠和豚鼠的心脏、骨骼肌、脾脏、肝脏、软骨、脑、神经、舌、食管和唾液腺的低温恒温器切片，用异硫氰酸荧光素标记的蓖麻凝集素和麦胚凝集素进行染色。染色后的切片用荧光显微镜检查。荧光的总体模式与用标记的菜豆凝集素和伴刀豆球蛋白A获得的模式非常相似。特别是凝集素对器官的结缔组织结构进行染色。与其他凝集素相比，麦胚凝集素在这些结构中的染色强度较弱。