Roth J, Neupert G, Thoss K
Exp Pathol (Jena). 1975;10(5-6):309-17. doi: 10.1016/s0014-4908(75)80039-1.
The observation of BOREK et al. (1973) on nonagglutinability of transformed rat liver cells by Lens culinaris lectin and our ultrastructural findings of a greater mobility of the Lens culinaris lectin receptors on transformed rat liver cells as compared to normal rat liver cells (ROTH 1975) initiated the present agglutination experiments on liver cells with lectins. For agglutination assay the microhemadsorption technique after FURMANSKI et al. (1973) was used with exception of several tests on EDTA-detached cells. The transformed rat liver cells exhibited, in contrast to the findings of BOREK et al. (1973), a positive microhemadsorption with Lens culinaris lectin as well as with Concanavalin A, Ricinus communis lectin and wheat germ agglutinin whereas the normal rat liver cells became positive only after a brief trypsin treatment. The significance of the difference in agglutinability of rat liver cells with Lens culinaris lectin and the other lectins used is discussed with regard to the cell-cell interaction mediated by lectins.
博雷克等人（1973年）观察到转化的大鼠肝细胞不能被菜豆凝集素凝集，而我（罗斯，1975年）的超微结构研究结果显示，与正常大鼠肝细胞相比，转化的大鼠肝细胞上的菜豆凝集素受体具有更大的流动性。基于上述观察和研究结果，我们开展了本次用凝集素对肝细胞进行凝集实验。凝集实验采用了弗曼斯基等人（1973年）之后的微量血吸附技术，但对用乙二胺四乙酸（EDTA）分离的细胞进行的几次测试除外。与博雷克等人（1973年）的研究结果相反，转化的大鼠肝细胞在用菜豆凝集素以及刀豆球蛋白A、蓖麻凝集素和小麦胚芽凝集素进行测试时，呈现出阳性微量血吸附反应；而正常大鼠肝细胞仅在经过短暂的胰蛋白酶处理后才呈阳性。本文结合凝集素介导的细胞间相互作用，讨论了大鼠肝细胞对菜豆凝集素和其他所用凝集素的凝集性差异的意义。
