异硫氰酸荧光素标记的凝集素在糖类免疫组织化学检测中的应用。
The use of fluorescein isothiocyanate labelled lectins for immunohistochemical demonstration of saccharides.
作者信息
Thoss K, Roth J
出版信息
Exp Pathol (Jena). 1975;11(3-4):155-61. doi: 10.1016/s0014-4908(75)80056-1.
Cryostat sections of various tissues from man, rat, mice, hamster, rabbit, guinea pig, sparrow and carp were examined by fluorescence microscopy after incubation with fluorescein isothiocyanate labelled Concanavalin A and Lens culinaris lectin. In the same tissue of various species no basic differences in the pattern of fluorescence were observed. In general, connective tissue structures were stained by both lectins in the same degree, whereas by fluorescein isothiocyanate labelled Concanavalin A additionally a varying staining of the cell surface and of cytoplasmic structures occurred. The results show the general presence of Concanavalin A and Lens culinaris lectin receptors in normal animal tissues.
将异硫氰酸荧光素标记的伴刀豆球蛋白A和菜豆凝集素与来自人、大鼠、小鼠、仓鼠、兔子、豚鼠、麻雀和鲤鱼的各种组织的低温恒温器切片一起孵育后，通过荧光显微镜进行检查。在不同物种的相同组织中，未观察到荧光模式的基本差异。一般来说，两种凝集素对结缔组织结构的染色程度相同，而异硫氰酸荧光素标记的伴刀豆球蛋白A还会使细胞表面和细胞质结构出现不同程度的染色。结果表明正常动物组织中普遍存在伴刀豆球蛋白A和菜豆凝集素受体。
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