Jentzsch K D, Schnitzler S, Mauersberger B, Jacob W
Exp Pathol (Jena). 1977 Sep-Oct;14(3-4):220-6. doi: 10.1016/s0014-4908(77)80070-7.
By means of fluorescein-labeled anti-AHP agglutinin isolated from the albumen gland of the snail Helix pomatia L. L cells were detected in air dried and fixed monolayer preparations, smear preparations or cryostat sections of tumours induced in two different host systems. The tumours consisting of L cells could be clearly distinguished from the surrounding normal host tissues, and also single tumour cells lying separtely in the host tissued were identified in the same unequivocal manner. The cells were marked by characteristic granular fluorescence caused by specific agglutinin binding within the cell membrane. With these results the previously expressed thesis that anti-AHP agglutinin might be used as a marker for L cells is confirmed for L cells grown in suitable host systems, too.
利用从罗马蜗牛蛋白腺中分离出的荧光素标记抗AHP凝集素，在两种不同宿主系统中诱导产生的肿瘤的空气干燥固定单层制剂、涂片制剂或低温切片中检测到L细胞。由L细胞组成的肿瘤可与周围正常宿主组织清楚区分，并且以同样明确的方式识别出单独位于宿主组织中的单个肿瘤细胞。这些细胞通过细胞膜内特异性凝集素结合产生的特征性颗粒荧光进行标记。这些结果证实，对于在合适宿主系统中生长的L细胞，之前提出的抗AHP凝集素可作为L细胞标记物的论点也是成立的。
