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利用来自苹果螺的金标凝集素通过电子显微镜检测人红细胞上的隐抗原AhumHP(弗里德赖希抗原)。

Electron microscopic detection of the cryptantigen AhumHP (Friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from Helix pomatia.

作者信息

Wagner M, Wagner B

出版信息

Z Immunitatsforsch Immunobiol. 1977 Dec;153(5):450-6.

PMID:602347
Abstract

Using the gold-labelled agglutinin anti-AHP from Helix pomatia (edible snail) the ultrastructural site and distribution of the cryptantigen (Friedenreich antigen) AhumHP was revealed on neuraminidase treated human O and B erythrocytes. On A1 and A2 cells the antigen was demonstrated simultaneously with the blood group antigen A by labelling the cells with anti-AHP-ferritin followed by removing the neuraminic acid and labelling with anti-AHP-gold. The cryptantigen is focally distributed on the cell surface.

摘要

使用来自食用蜗牛(苹果螺)的金标记抗AHP凝集素,在经神经氨酸酶处理的人O型和B型红细胞上揭示了隐抗原(弗里德赖希抗原)AhumHP的超微结构位点和分布。在A1型和A2型细胞上,通过用抗AHP-铁蛋白标记细胞,然后去除神经氨酸并用抗AHP-金标记,同时证明了该抗原与血型抗原A。隐抗原在细胞表面呈局灶性分布。

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1
Electron microscopic detection of the cryptantigen AhumHP (Friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from Helix pomatia.利用来自苹果螺的金标凝集素通过电子显微镜检测人红细胞上的隐抗原AhumHP(弗里德赖希抗原)。
Z Immunitatsforsch Immunobiol. 1977 Dec;153(5):450-6.
2
[Electron microscopic detection of blood group antigen A on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia].[利用铁蛋白和金标记的苹果螺保护素对人红细胞血型抗原A进行电子显微镜检测]
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[Investigations about an alleged lysozym-like behavior of the protectine anti-AHP from the albumen gland of the edible snail (Helix pomatia) (author's transl)].关于食用蜗牛(苹果螺)蛋白腺中保护素抗AHP类似溶菌酶行为的研究(作者译)
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