Mao Haiguang, Xu Xiuli, Cao Haiyue, Dong Xinyang, Zou Xiaoting, Xu Ningying, Yin Zhaozheng
Animal Science College, Zhejiang University, Hangzhou, Zhejiang, China.
School of Biological and Chemical Engineering, Ningbo Tech University, Ningbo, Zhejiang, China.
Front Genet. 2021 Mar 23;12:571325. doi: 10.3389/fgene.2021.571325. eCollection 2021.
Egg production performance is one of the most important economic traits in pigeon industry. However, little is known regarding how egg production performance is regulated by long non-coding RNAs (lncRNAs) in pigeons. To evaluate the lncRNAs and mRNAs in ovaries associated with egg production performance in domestic pigeons, high-throughput RNA sequencing of ovaries between high and low egg production performance groups were performed and analyzed in this study. A total of 34,346 mRNAs and 24,601 lncRNAs were identified, including 14,525 known lncRNAs and 10,076 novel lncRNAs, of which 811 mRNAs and 148 lncRNAs ( < 0.05) were significantly differentially expressed (DE) between the groups of high and low egg production performance. GO and KEGG annotation analysis indicated that the target genes of DE lncRNAs and DE mRNAs were related to cell differentiation, ATP binding and methylation. Moreover, we found that FOXK2, a target gene of lncRNA MSTRG.7894.4, was involved in regulating estrogen receptors. Our study provided a catalog of lncRNAs and mRNAs associated with egg production performance, and they deserve further study to deepen the understanding of biological processes in the ovaries of pigeons.
产蛋性能是鸽业中最重要的经济性状之一。然而，关于长链非编码RNA（lncRNA）如何调控鸽子的产蛋性能，人们了解甚少。为了评估家鸽卵巢中与产蛋性能相关的lncRNA和mRNA，本研究对高产蛋性能组和低产蛋性能组的卵巢进行了高通量RNA测序并分析。共鉴定出34346个mRNA和24601个lncRNA, 其中包括14525个已知lncRNA和10076个新lncRNA，在高产蛋性能组和低产蛋性能组之间，有811个mRNA和148个lncRNA（<0.05）显著差异表达（DE）。GO和KEGG注释分析表明，差异表达lncRNA和差异表达mRNA的靶基因与细胞分化、ATP结合和甲基化有关。此外，我们发现lncRNA MSTRG.7894.4的靶基因FOXK2参与调节雌激素受体。我们的研究提供了一份与产蛋性能相关的lncRNA和mRNA目录，它们值得进一步研究，以加深对鸽卵巢生物学过程的理解。
