Yin Zhaozheng, Dong Xinyang, Ma Youzhi, Dong Dingjuan
Animal Science College, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
J Poult Sci. 2017 Jan 25;54(1):13-17. doi: 10.2141/jpsa.0160037.
Dopamine inhibited prolactin secretion via dopamine D2 receptor (DRD2) at the pituitary level, but its effects on reproduction in pigeons are unclear. In this study, Single Nucleotide Polymorphisms (SNPs) in the exons of DRD2 gene were identified and analyzed by using DNA sequencing methods in 60 female domestic pigeons (), and the association between DRD2 polymorphisms and reproduction traits was also analyzed. Sequencing results showed that 7 nucleotide mutations were detected in the exon 1, 4, and 6 regions of DRD2 gene. The analysis revealed three genotypes (AA, AB, and BB) in exon 4 and two genotypes (AA, AB) in exon 6, in which the AA genotype was consistently dominant, and the A allele showed a dominant advantage. The C4532T genotypes located in exon 6 of DRD2 gene were significantly (<0.05) associated with reproductive traits of pigeon. Moreover, the individuals with AB genotype had significantly higher fertility rate and total hatching number within 500 days of age than those with AA genotype (<0.05). These findings suggested that the DRD2 gene should be included in future genetic studies of pigeon reproduction and the SNP of C4532T might be a potential candidate genetic marker for Marker-aid breeding in pigeon.
多巴胺在垂体水平通过多巴胺D2受体（DRD2）抑制催乳素分泌，但其对鸽子繁殖的影响尚不清楚。在本研究中，采用DNA测序方法对60只雌性家鸽DRD2基因外显子中的单核苷酸多态性（SNP）进行了鉴定和分析，并分析了DRD2多态性与繁殖性状之间的关联。测序结果显示，在DRD2基因的外显子1、4和6区域检测到7个核苷酸突变。分析发现外显子4中有三种基因型（AA、AB和BB），外显子6中有两种基因型（AA、AB），其中AA基因型始终占主导地位，A等位基因显示出显性优势。位于DRD2基因外显子6的C4532T基因型与鸽子的繁殖性状显著相关（<0.05）。此外，AB基因型个体在500日龄内的受精率和总孵化数显著高于AA基因型个体（<0.05）。这些发现表明，DRD2基因应纳入未来鸽子繁殖的遗传研究中，C4532T的SNP可能是鸽子标记辅助育种的潜在候选遗传标记。
