Blaschek W, Hess D
Experientia. 1977 Dec 15;33(12):1595-6. doi: 10.1007/BF01934017.
Plant nuclei prepared from protoplasts can be used as a cell-free system for testing their template activity of procaryotic DNA for plant polymerases. We were able to demonstrate that plant polymerases of Petunia hybrida are capable of transcribing linear bacterial DNA, whereas supercoiled DNA could not be used as a template.
从原生质体中制备的植物细胞核可作为无细胞系统,用于测试原核DNA对植物聚合酶的模板活性。我们能够证明,矮牵牛的植物聚合酶能够转录线性细菌DNA,而超螺旋DNA不能用作模板。