2型糖尿病患者载脂蛋白E基因多态性与血脂谱及缺血性卒中风险的关联

Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients.

作者信息

Maratni Ni Putu Tesi, Saraswati Made Ratna, Dewi Ni Nyoman Ayu, Yasa I Wayan Putu Sutirta, Eka Widyadharma I Putu, Putra Ida Bagus Kusuma, Suastika Ketut

机构信息

Doctoral Program of Medical Sciences, Faculty of Medicine, Udayana University, Bali, Indonesia.

Department of Internal Medicine, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, Indonesia.

出版信息

J Nutr Metab. 2021 Mar 26;2021:5527736. doi: 10.1155/2021/5527736. eCollection 2021.

DOI:10.1155/2021/5527736

PMID:33833872

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8018876/

Abstract

BACKGROUND

Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. This research aimed to determine the association of apolipoprotein E gene polymorphism with lipid profile and IS risk in T2DM patients.

METHODS

This case-control study involved a total of 60 diabetic participants divided into two groups with and without IS. ApoE was genotyped using PCR and sequencing analysis.

RESULTS

The most predominant genotype observed in 27 participants (45%) was E3/E3. Lower levels of high-density lipoprotein cholesterol (HDL-C) were found in 2 carriers (=0.003; 95% CI -23.35--4.89) and 4 carriers (=0.019; 95% CI 1.38-14.55) compared to 3 homozygotes. Total cholesterol (TC), triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels had no association with ApoE gene polymorphism in this study. ApoE gene polymorphism was not related to IS in T2DM (=0.06; adjusted OR: 4.71; 95% CI 0.93-23.79).

CONCLUSIONS

ApoE 2 and 4 carriers were associated with lower levels of HDL-C. No association was identified between ApoE gene polymorphism and IS in T2DM patients.

摘要

背景

脂质谱改变一直与脑血管事件有关。缺血性中风（IS）是2型糖尿病（T2DM）中常见的合并症。载脂蛋白E（ApoE）基因在脂蛋白代谢中具有显著关键作用，被认为是T2DM中易患IS并发症的潜在候选基因之一。本研究旨在确定载脂蛋白E基因多态性与T2DM患者脂质谱及IS风险之间的关联。

方法

本病例对照研究共纳入60名糖尿病参与者，分为有IS和无IS两组。采用聚合酶链反应（PCR）和测序分析对ApoE进行基因分型。

结果

在27名参与者（45%）中观察到最主要的基因型为E3/E3。与3名纯合子相比，2名E2携带者（P=0.003；95%可信区间-23.35--4.89）和4名E4携带者（P=0.019；95%可信区间1.38-14.55）的高密度脂蛋白胆固醇（HDL-C）水平较低。本研究中，总胆固醇（TC）、甘油三酯和低密度脂蛋白胆固醇（LDL-C）水平与ApoE基因多态性无关。ApoE基因多态性与T2DM中的IS无关（P=0.06；校正比值比：4.71；95%可信区间0.93-23.79）。