Wiechmann Anika, Wilde Benjamin, Tyczynski Bartosz, Amann Kerstin, Abdulahad Wayel H, Kribben Andreas, Lang Karl Sebastian, Witzke Oliver, Dolff Sebastian
Department of Nephrology, University Duisburg-Essen, Essen, Germany.
Department of Medical Intensive Care I, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Front Med (Lausanne). 2021 Mar 23;8:556776. doi: 10.3389/fmed.2021.556776. eCollection 2021.
Cytotoxic CD8 T-cells play a pivotal role in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to investigate the role of CD107a (LAMP-1) on cytotoxic CD8 T-cells in SLE-patients in particular with lupus nephritis. Peripheral blood of SLE-patients ( = 31) and healthy controls ( = 21) was analyzed for the expression of CD314 and CD107a by flow cytometry. Kidney biopsies of lupus nephritis patients were investigated for the presence of CD8 and C107a cells by immunohistochemistry and immunofluorescence staining. The percentages of CD107a on CD8 T-cells were significantly decreased in SLE-patients as compared to healthy controls (40.2 ± 18.5% vs. 47.9 ± 15.0%, = 0.02). This was even more significant in SLE-patients with inactive disease. There was a significant correlation between the percentages of CD107aCD8 T-cells and SLEDAI. The evaluation of lupus nephritis biopsies showed a significant number of CD107aCD8 T-cells mainly located in the peritubular infiltrates. The intrarenal expression of CD107a was significantly correlated with proteinuria. These results demonstrate that CD8 T-cells of patients with systemic lupus erythematosus have an altered expression of CD107a which seems to be associated with disease activity. The proof of intrarenal CD107aCD8 suggests a role in the pathogenesis of lupus nephritis.
细胞毒性CD8 T细胞在系统性红斑狼疮（SLE）的发病机制中起关键作用。本研究的目的是调查CD107a（溶酶体相关膜蛋白1）在SLE患者尤其是狼疮性肾炎患者的细胞毒性CD8 T细胞中的作用。通过流式细胞术分析SLE患者（n = 31）和健康对照者（n = 21）外周血中CD314和CD107a的表达。通过免疫组织化学和免疫荧光染色研究狼疮性肾炎患者肾活检组织中CD8和C107a细胞的存在情况。与健康对照者相比，SLE患者CD8 T细胞上CD107a的百分比显著降低（40.2±18.5%对47.9±15.0%，P = 0.02）。在疾病不活动的SLE患者中这种情况甚至更明显。CD107a+CD8 T细胞的百分比与SLE疾病活动指数（SLEDAI）之间存在显著相关性。狼疮性肾炎活检组织的评估显示大量CD107a+CD8 T细胞主要位于肾小管周围浸润处。肾内CD107a的表达与蛋白尿显著相关。这些结果表明系统性红斑狼疮患者的CD8 T细胞CD107a表达改变，这似乎与疾病活动有关。肾内CD107a+CD8的证实提示其在狼疮性肾炎发病机制中的作用。
