[系统性红斑狼疮患者外周血中CD4(+)和CD8(+) T细胞上PD-1的表达及意义]

[Expression and significance of PD-1 on CD4(+); and CD8(+); T cells from the peripheral blood of patients with systemic lupus erythematosus].

作者信息

Wang Yuanyuan, Xie Changhao, Sun Meiqun, Chai Jixia, Zhou Yanmei, Chen Yong, Tang Jie, Li Boqing

机构信息

Department of Histology and Embryology, Bengbu Medical College, Bengbu, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Sep;29(9):962-5.

DOI:

PMID:24011159

Abstract

OBJECTIVE

To detect the expression of programmed death-1 (PD-1) on CD4(+); and CD8(+); T cells from the peripheral blood of patients with systemic lupus erythematosus (SLE) and analyze its clinical implications.

METHODS

The expression of PD-1 on the CD4(+); and CD8(+); T cells were examined in 51 SLE patients and 38 healthy controls (HCs) by flow cytometry. The proportions of PD-1 positive CD4(+); and CD8(+); T cells were compared not only between inactive or active SLE patients and HCs, but also between patients with and without lupus nephritis. The correlations of PD-1 expression with clinical manifestations and laboratory findings were analyzed.

RESULTS

The proportions of CD4(+); PD-1(+); T cells significantly increased in active SLE patients as compared with HCs and inactive SLE ones (all P<0.05). The proportions of CD8(+); PD-1(+); T cells were significantly higher in active and inactive SLE patients than those in HCs (all P<0.05). The numbers of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells in SLE patients with nephritis were significantly higher than those in patients without nephritis (P<0.01). In SLE patients, the numbers of CD4(+);PD-1(+); and CD8(+);PD-1(+); T cells were significantly higher in ones with positive anti-dsDNA, anti-Sm, and anti-nucleosome antibodies than in corresponding negative ones; the proportions of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were positively correlated with SLEDAI score and the amount of proteinuria, but were negatively correlated with complement C3.

CONCLUSION

The aberrations of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were observed in patients with SLE. Increased numbers of CD4(+); PD-1(+); and CD8(+); PD-1(+); T cells were correlated with increased disease activity and the production of anti-self antibodies.

摘要

目的

检测系统性红斑狼疮（SLE）患者外周血CD4⁺和CD8⁺T细胞上程序性死亡蛋白1（PD - 1）的表达，并分析其临床意义。

方法

采用流式细胞术检测51例SLE患者和38例健康对照（HCs）外周血CD4⁺和CD8⁺T细胞上PD - 1的表达。比较非活动期或活动期SLE患者与HCs之间，以及有或无狼疮肾炎患者之间PD - 1阳性CD4⁺和CD8⁺T细胞的比例。分析PD - 1表达与临床表现及实验室检查结果的相关性。

结果

与HCs及非活动期SLE患者相比，活动期SLE患者CD4⁺PD - 1⁺T细胞比例显著升高（均P<0.05）。活动期和非活动期SLE患者CD8⁺PD - 1⁺T细胞比例均显著高于HCs（均P<0.05）。有肾炎的SLE患者CD4⁺PD - 1⁺和CD8⁺PD - 1⁺T细胞数量显著高于无肾炎患者（P<0.01）。在SLE患者中，抗双链DNA、抗Sm和抗核小体抗体阳性者的CD4⁺PD - 1⁺和CD8⁺PD - 1⁺T细胞数量显著高于相应抗体阴性者；CD4⁺PD - 1⁺和CD8⁺PD - 1⁺T细胞比例与SLE疾病活动指数（SLEDAI）评分及蛋白尿定量呈正相关，与补体C3呈负相关。