Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.
Appl Biochem Biotechnol. 2021 Sep;193(9):2759-2768. doi: 10.1007/s12010-021-03563-3. Epub 2021 Apr 8.
Ketone bodies (acetoacetate, beta-hydroxybutyrate (βHB), acetone) are generated as a result of fatty acid oxidation in the liver and exist at low concentrations in urine and blood. Elevated concentrations can indicate health problems such as diabetes, childhood hypoglycemia, alcohol, or salicylate poisoning. Development of portable and cost-effective bedside point-of-care (POC) tests to detect such compounds can help to reduce the risk of disease progression. In this study, βHB was chosen as a model molecule for developing an optical sensor-integrated microplatform. Prior to sensor optimization, βHB levels were measured at a concentration range of 0.02 and 0.1 mM spectrophotometrically, which is far below the reported elevated ranges of 1-2 mM and resulting absorbance changes were converted into an Arduino microcontroller code for the correlation. Measurements performed with the designed integrated microplatform were found significant. Integrated microplatform was verified with the benchtop spectrophotometer. Measurements between 0.02 and 0.1 mM substrate concentration were found highly sensitive with "y = 0.7347x + 0.00184" with R value of 0.9796, and the limit of detection was determined as 0.02 mM. Based on these results, the proposed system will allow on-site and early intervention.
酮体（乙酰乙酸盐、β-羟丁酸（βHB）、丙酮）是肝脏脂肪酸氧化的结果，在尿液和血液中的浓度较低。浓度升高可能表明存在健康问题，如糖尿病、儿童低血糖、酒精或水杨酸盐中毒。开发便携式且具有成本效益的床边即时护理（POC）检测点测试，以检测此类化合物，可以帮助降低疾病进展的风险。在这项研究中，βHB 被选为开发光学传感器集成微平台的模型分子。在传感器优化之前，使用分光光度法在 0.02 和 0.1 mM 的浓度范围内测量 βHB 水平，这远低于报道的 1-2 mM 的升高范围，并且将吸光度变化转换为用于相关性的 Arduino 微控制器代码。用设计的集成微平台进行的测量结果是显著的。用台式分光光度计对集成微平台进行了验证。在 0.02 和 0.1 mM 底物浓度之间的测量结果显示出高度的灵敏度，“y = 0.7347x + 0.00184”的 R 值为 0.9796，检测限确定为 0.02 mM。基于这些结果，建议的系统将允许现场和早期干预。
