School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
J Hazard Mater. 2021 Aug 15;416:125776. doi: 10.1016/j.jhazmat.2021.125776. Epub 2021 Mar 27.
In this research, an immobilized biofilm reactor was established for the simultaneous removal of calcium (Ca), fluoride (F), nickel (Ni), and nitrate (NO-N) by microbial induced calcium precipitation (MICP). The operating parameters of the reactor, hydraulic retention time (HRT: 4, 8, and 12 h), influent Ca concentration (36.0, 108.0, and 180.0 mg L), and influent Ni concentration (0.0, 3.0, and 6.0 mg L) were discussed. Under the HRT of 12 h, influent Ca concentration of 180.0 mg L, and influent Ni concentration of 3.0 mg L, the removal ratios of Ca, F, Ni, and NO-N reached 45.31%, 79.55%, 85.11%, and 55.29%, respectively, which was the reactor stable operation performance. The SEM revealed the morphology of calcium-precipitated bio-crystals. XPS showed the Ca and Ni precipitate components and XRD further revealed the formation of CaCO, Ca(PO)OH, and NiCO precipitation. Nitrogen (N) was the main gas produced in the reactor. Fluorescence spectroscopy manifested that extracellular polymers played an important role in the organism nucleation. High-throughput sequencing exhibited that Acinetobacter sp. H12 was the dominant bacterial group. This study provided a new insight for simultaneous remediation of Ca, F, Ni, and NO-N in water bodies.
在这项研究中，建立了固定化生物膜反应器，通过微生物诱导碳酸钙沉淀（MICP）同时去除钙（Ca）、氟（F）、镍（Ni）和硝酸盐（NO-N）。讨论了反应器的操作参数，水力停留时间（HRT：4、8 和 12 h）、进水 Ca 浓度（36.0、108.0 和 180.0 mg/L）和进水 Ni 浓度（0.0、3.0 和 6.0 mg/L）。在 HRT 为 12 h、进水 Ca 浓度为 180.0 mg/L、进水 Ni 浓度为 3.0 mg/L 的条件下，Ca、F、Ni 和 NO-N 的去除率分别达到 45.31%、79.55%、85.11%和 55.29%，这是反应器稳定运行的性能。SEM 揭示了钙沉淀生物晶体的形态。XPS 显示了 Ca 和 Ni 沉淀物的成分，XRD 进一步揭示了 CaCO、Ca（PO）OH 和 NiCO 沉淀的形成。氮气（N）是反应器中主要产生的气体。荧光光谱表明，胞外聚合物在生物机体成核过程中发挥了重要作用。高通量测序表明，不动杆菌属 H12 是主要的细菌群体。本研究为水体中 Ca、F、Ni 和 NO-N 的同时修复提供了新的思路。
