School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Chemosphere. 2022 Sep;302:134848. doi: 10.1016/j.chemosphere.2022.134848. Epub 2022 May 5.
Self-assembled fungus-flexible fiber composite microspheres (SFFMs) were firstly combined with microbially induced calcium precipitation (MICP) in a continuous-flow bioreactor and achieved the efficient removal of fluoride (F), nitrate (NO), and calcium (Ca). Under the influent F of 3.0 mg L, pH of 7.0, and HRT of 8 h, the average removal efficiencies reached 77.54%, 99.39%, and 67.25% (0.29, 2.03, and 8.34 mg L h), respectively. Fluorescence spectrum and flow cytometry analyses indicated that F content significantly affected the metabolism and viability of bacteria. SEM images showed that flexible fibers and intertwined hyphae provided effective locations for bacterial colonization in SFFMs. The precipitated products were characterized by XRD and FTIR, which revealed that F was mainly removed in the form of calcium fluoride and calcium fluorophosphate (CaF and Ca(PO)F). High-throughput analysis at different levels demonstrated that Pseudomonas sp. WZ39 acted as the core strain, which played a crucial role in the bioreactor. The mechanism of enhanced denitrification was attributed to minor F stress and bioaugmentation technology. This study highlighted the superiorities of SFFMs and MICP combined remediation and documented a promising option for F, NO, and Ca removal.
自组装真菌柔性纤维复合微球（SFFMs）首次在连续流生物反应器中与微生物诱导碳酸钙沉淀（MICP）结合，实现了氟化物（F）、硝酸盐（NO）和钙（Ca）的高效去除。在进水 F 为 3.0mg/L、pH 值为 7.0 和 HRT 为 8h 的条件下，平均去除效率分别达到 77.54%、99.39%和 67.25%（0.29、2.03 和 8.34mg/L·h）。荧光光谱和流式细胞术分析表明，F 含量显著影响细菌的代谢和活力。SEM 图像表明，柔性纤维和交织的菌丝为 SFFMs 中细菌定植提供了有效的位置。沉淀产物的 XRD 和 FTIR 特征表明，F 主要以氟化钙和氟磷酸钙（CaF 和 Ca(PO)F）的形式被去除。在不同水平的高通量分析表明，假单胞菌 WZ39 作为核心菌株，在生物反应器中发挥着关键作用。强化反硝化的机制归因于较小的 F 胁迫和生物增强技术。本研究强调了 SFFMs 和 MICP 联合修复的优越性，并为 F、NO 和 Ca 的去除提供了一种有前途的选择。
