School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Bioresour Technol. 2021 Aug;333:125154. doi: 10.1016/j.biortech.2021.125154. Epub 2021 Apr 17.
This study investigated the influence of different carbon to nitrogen (C/N) ratios on the bio-removal efficiency of aquatic pollutants like calcium (Ca), fluoride (F), and nitrate (NO-N) in a quartz sand-filled biofilm reactor (QSBR) to treat the low C/N wastewater using Acinetobacter sp. H12 at pH 6.50. The simultaneous bio-removal rate of Ca, F, and NO reached 56.31%, 96.33, and 96.95 respectively. Nitrogen gas (N) was produced with no evidence of NO emission. Moreover, the morphological study of strain H12 and biological precipitates through SEM revealed that strain H12 provides the nucleation sites for microbially induced calcium precipitation to remove Ca and F. Besides, XPS and XRD peak spectra implicated that Ca and F were removed as CaF and Ca(PO)F co-precipitates. The 16S rRNA sequencing analyses revealed that H12 belongs to Acinetobacter and has stronger MICP and denitrification potential as compared with other strains under low C/N conditions.
本研究调查了不同碳氮比（C/N）对在 pH 值为 6.50 时使用不动杆菌属 H12 处理低 C/N 废水的石英砂填充生物膜反应器（QSBR）中水生污染物（如钙（Ca）、氟（F）和硝酸盐（NO-N））的生物去除效率的影响。Ca、F 和 NO 的同时生物去除率分别达到 56.31%、96.33%和 96.95%。没有证据表明有 NO 排放。此外，通过 SEM 对菌株 H12 和生物沉淀物的形态研究表明，菌株 H12 为微生物诱导的钙沉淀提供了成核位点，以去除 Ca 和 F。此外，XPS 和 XRD 峰谱表明 Ca 和 F 被去除为 CaF 和 Ca(PO)F 共沉淀物。16S rRNA 测序分析表明，与其他菌株相比，H12 属于不动杆菌属，在低 C/N 条件下具有更强的 MICP 和反硝化潜力。
