Molinnus Denise, Drinic Aleksander, Iken Heiko, Kröger Nadja, Zinser Max, Smeets Ralf, Köpf Marius, Kopp Alexander, Schöning Michael J
Institute of Nano- and Biotechnologies (INB), FH Aachen, Campus Jülich, Heinrich-Mußmann-Strasse 1, 52428, Jülich, Germany.
Fibrothelium GmbH, TRIWO Technopark Aachen, Philipsstr. 8, 52068, Aachen, Germany.
Biosens Bioelectron. 2021 Jul 1;183:113204. doi: 10.1016/j.bios.2021.113204. Epub 2021 Mar 28.
In modern days, there is an increasing relevance of and demand for flexible and biocompatible sensors for in-vivo and epidermal applications. One promising strategy is the implementation of biological (natural) polymers, which offer new opportunities for flexible biosensor devices due to their high biocompatibility and adjustable biodegradability. As a proof-of-concept experiment, a biosensor was fabricated by combining thin- (for Pt working- and counter electrode) and thick-film (for Ag/AgCl quasi-reference electrode) technologies: The biosensor consists of a fully bio-based and biodegradable fibroin substrate derived from silk fibroin of the silkworm Bombyx mori combined with immobilized enzyme glucose oxidase. The flexible glucose biosensor is encapsulated by a biocompatible silicon rubber which is certificated for a safe use onto human skin. Characterization of the sensor set-up is exemplarily demonstrated by glucose measurements in buffer and Ringer's solution, while the stability of the quasi-reference electrode has been investigated versus a commercial Ag/AgCl reference electrode. Repeated bending studies validated the mechanical properties of the electrode structures. The cross-sensitivity of the biosensor against ascorbic acid, noradrenaline and adrenaline was investigated, too. Additionally, biocompatibility and degradation tests of the silk fibroin with and without thin-film platinum electrodes were carried out.
在现代，用于体内和表皮应用的灵活且生物相容的传感器的相关性和需求日益增加。一种有前景的策略是采用生物（天然）聚合物，由于其高生物相容性和可调节的生物降解性，为灵活的生物传感器设备提供了新机遇。作为概念验证实验，通过结合薄膜（用于铂工作电极和对电极）和厚膜（用于Ag/AgCl准参比电极）技术制造了一种生物传感器：该生物传感器由源自家蚕（Bombyx mori）丝素蛋白的完全基于生物且可生物降解的丝素蛋白底物与固定化酶葡萄糖氧化酶组合而成。这种灵活的葡萄糖生物传感器由经认证可安全用于人体皮肤的生物相容性硅橡胶封装。通过在缓冲液和林格氏溶液中进行葡萄糖测量示例性地展示了传感器设置的表征，同时针对商业Ag/AgCl参比电极研究了准参比电极的稳定性。重复弯曲研究验证了电极结构的机械性能。还研究了该生物传感器对抗坏血酸、去甲肾上腺素和肾上腺素的交叉敏感性。此外，对有无薄膜铂电极的丝素蛋白进行了生物相容性和降解测试。
