National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
J Pharm Biomed Anal. 2021 May 30;199:114036. doi: 10.1016/j.jpba.2021.114036. Epub 2021 Mar 23.
Stem and leaf of Cissus quadrangularis L. (Vitaceae), indigenous to Asia and Africa, were used for medicinal and dietary purposes with limited information about the plant's phytochemistry. Stem and leaf samples were assessed for the simultaneous determination of polyphenolic compounds (catechin, epicatechin, quercetin-3-O-β-glucopyranoside, kaempferol-3-O-β-glucoside, quercetin-3-O-β-rhamnoside, leachianol F, amurensin A, pallidol, resveratrol, and quadrangularin A), using UHPLC-PDA-MS. The validation data showed that the method is precise, specific, accurate, and linear over the range of 0.5-100 μg/mL. Reversed-phase ultra-high performance liquid chromatography (UHPLC) fingerprints of the crude methanolic stem and leaf extracts of C. quadrangularis were obtained at different wavelengths based on their λ. Polyphenolics were characterized using both UHPLC-PDA-MS and LC-QToF analysis. From liquid chromatography quadrupole time of flight-electrospray ionization mass spectrometry (LC-QToF) spectra, over 40 components were structurally correlated, and confirmation was based on the fragmentation characteristics and also from the information available in the literature. In addition to the LC-QToF method, a simple, fast HPTLC method was developed as a visual aid for the rapid qualitative analytical tool to help establish the quality assessment of botanical raw materials and dietary supplements claiming to contain Cissus.
蔓九节（葡萄科）的茎和叶，原产于亚洲和非洲，被用于药用和食用目的，但关于该植物的植物化学信息有限。对茎和叶样品进行了多酚化合物（儿茶素、表儿茶素、槲皮素-3-O-β-吡喃葡萄糖苷、山柰酚-3-O-β-葡萄糖苷、槲皮素-3-O-β-鼠李糖苷、leachianol F、amurensin A、pallidol、白藜芦醇和 quadrangularin A）的同时测定，采用 UHPLC-PDA-MS。验证数据表明，该方法在 0.5-100μg/mL 范围内精确、特异、准确且线性。基于 λ，获得了蔓九节粗甲醇提取物的反相超高效液相色谱（UHPLC）指纹图谱。采用 UHPLC-PDA-MS 和 LC-QToF 分析对多酚进行了表征。从液相色谱-四极杆飞行时间-电喷雾电离质谱（LC-QToF）谱中，对 40 多个成分进行了结构相关性分析，并基于碎片特征和文献中提供的信息进行了确认。除了 LC-QToF 方法外，还开发了一种简单、快速的 HPTLC 方法，作为快速定性分析工具的视觉辅助，以帮助建立声称含有蔓九节的植物原料和膳食补充剂的质量评估。
