Guemmogne Temdie Romeo Joel, Ymele Chiogo Vouffo Edwige, Tietcheu Galani Borris Rosnay, Dabole Ladane Bertrand, Bianzoumbe Josee, Kuum Minoue Marc Germain, Ntchapda Fidèle, Dimo Theophile
Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, Ngaoundéré, Cameroon.
Food and Drug Administration, Center for Drug Evaluation and Research, Office of New Drugs, Silver Spring, USA.
Curr Ther Res Clin Exp. 2023 Sep 30;99:100716. doi: 10.1016/j.curtheres.2023.100716. eCollection 2023.
Acetaminophen-induced liver injury remains a significant public health problem because available treatments are limited due to their adverse effects. Medicinal plants, which are an important source of bioactive molecules, could be an alternative treatment for liver disease.
This study was designed to investigate the curative effect of aqueous extracts of (Vitaceae) and (Euphorbiaceae) on acetaminophen-induced liver injury in mice.
Mice were divided into groups and treated with distilled water, silymarin (50 mg/kg), a reference hepatoprotective agent, and aqueous extracts of and (50 and 100 mg/kg, PO, respectively). These substances were given as a single daily dose 4 hours after acetaminophen administration (300 mg/kg, PO) for 2 days. Mice were humanely put to death 24 hours after the last dose and serum alanine aminotransferase and aspartate aminotransferase activities, total bilirubin and protein levels, reduced glutathione, superoxide dismutase, malondialdehyde, catalase, and nitrite tissue levels were assessed. Histology of the livers of the mice was performed by hematoxylin and eosin staining.
Acetaminophen administration induced a significant ( < 0.05) mean (SEM) body weight loss (-14.45% [5.92%]), a significant elevation of alanine aminotransferase activity (15.08%), total protein and bilirubin levels (25.80%), and a significant ( < 0.05) increase in liver superoxide dismutase (67.71%), catalase (63.00%), glutathione (40.29%), malondialdehyde (30.67%), and nitrite levels compared with the control group. In curative treatment, and (50 and 100 mg/kg) significantly ( < 0.05) reduced mean (SEM) body weight loss (16.67% [7.16%] and 1.25% [0.51%], respectively), serum alanine aminotransferase activity (17.62% and 11.14%, respectively), bilirubin level (29.62% and 49.14%, respectively) compared with acetaminophen group, and normalized serum total protein level. Both extracts significantly ( < 0.05) reduced the levels of glutathione and malondialdehyde and normalized that of nitrite, superoxide dismutase, and catalase compared with the acetaminophen group. Hepatocyte necrosis and inflammatory cell infiltration were remarkably reduced by the plant extracts.
The results obtained are evidence in favor of the development of a formulation based on the extracts of these plants against liver diseases.
对乙酰氨基酚诱导的肝损伤仍然是一个重大的公共卫生问题，因为现有治疗方法因其不良反应而受到限制。药用植物是生物活性分子的重要来源，可能是肝病的替代治疗方法。
本研究旨在探讨葡萄科（Vitaceae）和大戟科（Euphorbiaceae）植物水提取物对乙酰氨基酚诱导的小鼠肝损伤的治疗效果。
将小鼠分组，分别用蒸馏水、水飞蓟宾（50 mg/kg）（一种参考保肝剂）以及葡萄科和大戟科植物水提取物（分别为50和100 mg/kg，口服给药）进行处理。在给予对乙酰氨基酚（300 mg/kg，口服给药）4小时后，每天给予这些物质一次，持续2天。在最后一剂给药24小时后，对小鼠实施安乐死，并评估血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性、总胆红素和蛋白质水平、还原型谷胱甘肽、超氧化物歧化酶、丙二醛、过氧化氢酶以及组织中亚硝酸盐水平。通过苏木精和伊红染色对小鼠肝脏进行组织学检查。
与对照组相比，给予对乙酰氨基酚导致显著（P<0.05）的平均（标准误）体重减轻（-14.45%[5.92%]）、丙氨酸氨基转移酶活性显著升高（15.08%）、总蛋白和胆红素水平显著升高（25.80%），并且肝脏中超氧化物歧化酶（67.71%）、过氧化氢酶（63.00%）、谷胱甘肽（40.29%）、丙二醛（30.67%）和亚硝酸盐水平显著（P<0.05）升高。在治疗性处理中，葡萄科和大戟科植物提取物（50和100 mg/kg）与对乙酰氨基酚组相比，显著（P<0.05）降低了平均（标准误）体重减轻（分别为16.67%[7.16%]和1.25%[0.51%]）、血清丙氨酸氨基转移酶活性（分别为17.62%和11.14%）、胆红素水平（分别为29.62%和49.14%），并且葡萄科植物提取物使血清总蛋白水平恢复正常。与对乙酰氨基酚组相比，两种提取物均显著（P<0.05）降低了谷胱甘肽和丙二醛水平，并使亚硝酸盐、超氧化物歧化酶和过氧化氢酶水平恢复正常。植物提取物显著减少了肝细胞坏死和炎性细胞浸润。
所获得的结果证明开发基于这些植物提取物的抗肝病制剂是有依据的。
