Department of Orthopaedic Surgery, RWTH Aachen University Clinic, Pauwelsstraße 30, 52074, Aachen, Germany.
BMC Genomics. 2021 Apr 10;22(1):254. doi: 10.1186/s12864-021-07584-4.
The differentiation of bone marrow mesenchymal stem cells is a complex and dynamic process. The gene expression pattern and mechanism of different periods of adipogenic and osteogenic differentiation remain unclear. Additionally, the interaction between these two lineage determination requires further exploration.
Five modules that were most significantly associated with osteogenic or adipogenic differentiation of BMSCs were selected for further investigation. Biological terms (e.g. ribosome biogenesis, TNF-α signalling pathway, glucose import and fatty acid metabolism) along with hub transcription factors (e.g. PPARG and YY1) and hub miRNAs (e.g. hsa-mir-26b-5p) were enriched in different modules. The expression pattern of 6 hub genes, ADIPOQ, FABP4, SLC7A5, SELPLG, BIRC3, and KLHL30 was validated by RT-qPCR. Finally, cell staining experiments extended the findings of bioinformatics analysis.
This study identified the key genes, biological functions, and regulators of each time point of adipogenic and osteogenic differentiation of BMSCs and provided novel evidence and ideas for further research on the differentiation of BMSCs.
骨髓间充质干细胞的分化是一个复杂而动态的过程。成脂和成骨分化不同时期的基因表达模式和机制尚不清楚。此外，这两个谱系决定之间的相互作用需要进一步探索。
选择与 BMSCs 成脂和成骨分化最显著相关的 5 个模块进行进一步研究。生物术语（如核糖体生物发生、TNF-α 信号通路、葡萄糖摄取和脂肪酸代谢）以及枢纽转录因子（如 PPARG 和 YY1）和枢纽 microRNA（如 hsa-mir-26b-5p）在不同模块中富集。通过 RT-qPCR 验证了 6 个枢纽基因 ADIPOQ、FABP4、SLC7A5、SELPLG、BIRC3 和 KLHL30 的表达模式。最后，细胞染色实验扩展了生物信息学分析的结果。
本研究鉴定了 BMSCs 成脂和成骨分化各时间点的关键基因、生物学功能和调控因子，为进一步研究 BMSCs 的分化提供了新的证据和思路。
