Peter the Great Saint Petersburg Polytechnic University, Polytekhnicheskaya str., 29, Saint Petersburg, 195251, Russia; Saint Petersburg State University of Industrial Technologies and Design, B. Morskaya str., 18, Saint Petersburg, 191186, Russia.
Peter the Great Saint Petersburg Polytechnic University, Polytekhnicheskaya str., 29, Saint Petersburg, 195251, Russia.
Carbohydr Polym. 2021 Jun 15;262:117917. doi: 10.1016/j.carbpol.2021.117917. Epub 2021 Mar 5.
The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies. It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.%. For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface. These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils. It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.
本文致力于研究壳聚糖基复合薄膜中壳聚糖的结构、表面形貌、机械性能和电导率对壳聚糖基复合薄膜的影响。研究结果表明，壳聚糖基复合薄膜的最佳纳米纤维浓度为 5wt%。在这种组成的薄膜中，我们观察到薄膜表面结构元素的取向，机械性能增强，以及薄膜表面皮肤成纤维细胞的比电导率和增殖活性增加。这些结果与纳米复合材料中出现的取向结构以及壳聚糖大分子在壳聚糖纳米纤维表面的自组织有关。研究表明，在复合薄膜表面培养过程中，表面能和表面亲水性的增加并没有促进细胞的有效黏附、活力和增殖活性。
