Liu Yongdi, Qian Yulei, Yong Xiaoyu, Jia Honghua, Wei Ping, Zhou Jun
Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Suzhou ViCheckBiotechnology Co, Ltd, Suzhou, Jiangsu 215000, China.
Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China.
Bioresour Technol. 2021 Jul;332:125109. doi: 10.1016/j.biortech.2021.125109. Epub 2021 Apr 5.
Anaerobic digestion (AD) systems with high substrate concentrations are characterized by high viscosity, which affects material and energy transfer efficiencies, thereby influencing methane production efficiency. In this study, adding granular activated carbon (GAC) and increasing the temperature decreased the viscosity by 4.56-10.19% and 27.13-28.85%, respectively, and improved AD efficiency. Adding GAC and increasing the temperature enhanced the methane yields by 34.37-38.15% and 25.60-28.31%, respectively. Distance-based redundancy analysis showed that the viscosity, temperature, and GAC had the greatest effects on the composition of the microbial community. The dominant bacteria in the medium-temperature AD system at the phylum level belonged to Firmicutes, Bacteroidetes, and Euryarchaeota. In addition to the dominant bacteria in the medium-temperature AD system, the thermophilic phylum Thermotogae was abundant in the high-temperature AD system. Moreover, the relative abundance of Euryarchaeota, which contained most of the methanogens, was higher in the high-temperature AD system than in the medium-temperature AD system.
高底物浓度的厌氧消化（AD）系统具有高粘度的特点，这会影响物质和能量的传递效率，进而影响甲烷生产效率。在本研究中，添加颗粒活性炭（GAC）和提高温度分别使粘度降低了4.56 - 10.19%和27.13 - 28.85%，并提高了AD效率。添加GAC和提高温度分别使甲烷产量提高了34.37 - 38.15%和25.60 - 28.31%。基于距离的冗余分析表明，粘度、温度和GAC对微生物群落组成的影响最大。中温AD系统中门水平的优势细菌属于厚壁菌门、拟杆菌门和广古菌门。除了中温AD系统中的优势细菌外，嗜热栖热菌门在高温AD系统中含量丰富。此外，包含大多数产甲烷菌的广古菌门在高温AD系统中的相对丰度高于中温AD系统。
