Koga Y, Sugimachi K, Kuwano H, Mori M, Matsufuji H
Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Eur J Cancer Clin Oncol. 1988 Apr;24(4):643-51. doi: 10.1016/0277-5379(88)90294-5.
A series of esophageal lesions, mild, moderate and severe dysplasia, carcinoma in situ (CIS), early and advanced squamous cell carcinoma were induced in rats with N-methyl-N-amylnitrosamine (MAN). To evaluate the biological nature of each lesion, the ploidy level was estimated by microspectrophotometrical measurement of cell nuclear DNA content. DNA distribution patterns were classified into types I, II, III and IV, according to the degree of dispersion and the peak modal value on the DNA histogram. The incidences of type III of high ploidy in early cancer, CIS and severe dysplasia were 3/11 (27.3%), 5/13 (33.3%) and 4/16 (25%), respectively. On the other hand, in moderate and mild dysplasia, 15/16 (93.8%) and 20/21 (95.2%) were low ploidy (types I and II), respectively. The mean DNA content of advanced and early cancer, CIS and severe dysplasia were 3.88c, 3.34c, 3.24c and 3.13c, respectively, while those of moderate and mild dysplasia were near diploid, showing 2.67c and 2.51c, respectively. These findings indicate that the biological nature of severe dysplasia may be considered as serious a lesion as cancer, in terms of DNA analysis. Cytophotometric DNA analysis aids the evaluation of various degrees of dysplasia and carcinoma of the esophagus.
用N-甲基-N-戊基亚硝胺（MAN）在大鼠中诱发了一系列食管病变，包括轻度、中度和重度发育异常、原位癌（CIS）、早期和晚期鳞状细胞癌。为了评估每个病变的生物学特性，通过细胞核DNA含量的显微分光光度测量来估计倍体水平。根据DNA直方图上的分散程度和峰值众数，DNA分布模式分为I、II、III和IV型。早期癌症、CIS和重度发育异常中高倍体III型的发生率分别为3/11（27.3%）、5/13（33.3%）和4/16（25%）。另一方面，在中度和轻度发育异常中，低倍体（I型和II型）分别为15/16（93.8%）和20/21（95.2%）。晚期和早期癌症、CIS和重度发育异常的平均DNA含量分别为3.88c、3.34c、3.24c和3.13c，而中度和轻度发育异常的平均DNA含量接近二倍体，分别为2.67c和2.51c。这些发现表明，从DNA分析的角度来看，重度发育异常的生物学特性可能被认为与癌症一样严重。细胞光度法DNA分析有助于评估食管不同程度的发育异常和癌症。
