Tan Phui Yee, Tan Tai Boon, Chang Hon Weng, Mwangi William W, Tey Beng Ti, Chan Eng Seng, Lai Oi Ming, Liu Yuanfa, Wang Yong, Tan Chin Ping
Department of Bioscience, Faculty of Applied Sciences, Tunku Abdul Rahman University College, Jalan Genting Kelang, Kuala Lumpur, 53300, Malaysia.
Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, 43400, Malaysia.
J Sci Food Agric. 2021 Nov;101(14):5963-5971. doi: 10.1002/jsfa.11249. Epub 2021 Apr 25.
Throughout the past decade, Pickering emulsion has been increasingly utilized for the encapsulation of bioactive compounds due to its high stability and biocompatibility. In the present work, palm tocotrienols were initially encapsulated in a calcium carbonate Pickering emulsion, which was then subjected to alginate gelation and subsequent chitosan coating. The effects of wall material (alginate and chitosan) concentrations, gelation pH and time, and chitosan coating time on the encapsulation efficiency of palm tocotrienols were explored.
Our findings revealed that uncoated alginate microcapsules ruptured upon drying and exhibited low encapsulation efficiency (13.81 ± 2.76%). However, the addition of chitosan successfully provided a more complex and rigid external wall structure to enhance the stability of the microcapsules. By prolonging the crosslinking time from 5 to 30 min and increasing the chitosan concentration from 0.1% to 0.5%, the oil encapsulation efficiency was increased by 28%. Under the right gelation pH (pH 4), the extension of gelation time from 1 to 12 h resulted in an increase in alginate-Ca crosslinkings, thus strengthening the microcapsules.
With the optimum formulation and process parameters, a high encapsulation efficiency (81.49 ± 1.75%) with an elevated oil loading efficiency (63.58 ± 2.96%) were achieved. The final product is biocompatible and can potentially be used for the delivery of palm tocotrienols. © 2021 Society of Chemical Industry.
在过去十年中，由于其高稳定性和生物相容性，皮克林乳液越来越多地用于生物活性化合物的包封。在本研究中，棕榈生育三烯酚最初被包封在碳酸钙皮克林乳液中，然后进行海藻酸钠凝胶化和随后的壳聚糖包衣。探讨了壁材（海藻酸钠和壳聚糖）浓度、凝胶化pH值和时间以及壳聚糖包衣时间对棕榈生育三烯酚包封效率的影响。
我们的研究结果表明，未包衣的海藻酸钠微胶囊在干燥时会破裂，包封效率较低（13.81±2.76%）。然而，壳聚糖的添加成功地提供了更复杂和刚性的外壁结构，以提高微胶囊的稳定性。通过将交联时间从5分钟延长到30分钟，并将壳聚糖浓度从0.1%提高到0.5%，油包封效率提高了28%。在合适的凝胶化pH值（pH 4）下，凝胶化时间从1小时延长到12小时会导致海藻酸钠-钙交联增加，从而增强微胶囊。
通过优化配方和工艺参数，实现了高包封效率（81.49±1.75%）和提高的油负载效率（63.58±2.96%）。最终产品具有生物相容性，可潜在地用于棕榈生育三烯酚的递送。©2021化学工业协会。
