Department of Food Technology, Engineering and Nutrition, Chemical Center, Lund University, Lund, Sweden.
Department of Clinical Sciences, Malmö, Surgery Research Unit and the Clinic of Surgery, Skåne University Hospital Malmö, Lund University, Malmö, Sweden.
Int J Food Sci Nutr. 2021 Dec;72(8):1071-1082. doi: 10.1080/09637486.2021.1906212. Epub 2021 Apr 11.
The number of elderlies is increasing but prevalence of malnutrition has been reported. The aim of the study was to determine the significance of short-term nutritional deficiencies in mice. Immune status was assessed through flow cytometry of leucocytes in Peyer's patches (PP) and mesenteric lymph nodes (MLN), and intestinal microbiota was evaluated by terminal restriction fragment length polymorphism (T-RFLP). C57BL/6NCrl mice fed standard diet (StD) or experimental diet high in fat, and low in carbohydrates, protein, fibre, vitamins, and minerals (ExpD) for 2 or 4 weeks. ExpD-animals gained less weight, increased liver lipids, and developed splenomegaly. Diet affected regulatory T-cells, gut homing receptors and TLR2 and TLR4 in PP and MLN and the microbiota was influenced. Partial least squares models on flow cytometry- and T-RFLP data demonstrated correlations between microbial communities and immune phenotyping. Our model shows similarities to malnourished elderly and interactions between intestinal bacteria and the immune system.
老年人数量不断增加，但营养不良的患病率却有所报道。本研究旨在确定短期营养缺乏对小鼠的影响。通过对派尔集合淋巴结（PP）和肠系膜淋巴结（MLN）中的白细胞进行流式细胞术来评估免疫状态，并通过末端限制性片段长度多态性（T-RFLP）评估肠道微生物群。将 C57BL/6NCrl 小鼠喂食标准饮食（StD）或高脂肪、低碳水化合物、低蛋白质、低纤维、低维生素和低矿物质的实验饮食（ExpD）2 或 4 周。ExpD 喂养的动物体重增加较少，肝脏脂质增加，并出现脾肿大。饮食影响了 PP 和 MLN 中的调节性 T 细胞、肠道归巢受体以及 TLR2 和 TLR4，同时也影响了微生物群。基于流式细胞术和 T-RFLP 数据的偏最小二乘模型显示了微生物群落与免疫表型之间的相关性。我们的模型与营养不良的老年人相似，并且肠道细菌与免疫系统之间存在相互作用。
