钙和温度对混合脂质-缬氨霉素单层膜的影响。糖鞘脂（神经节苷脂GT1b、硫脂）与磷脂酰胆碱的比较。

Effect of calcium and temperature on mixed lipid-valinomycin monolayers. A comparison of glycosphingolipids (ganglioside GT1b, sulphatides) and phosphatidylcholine.

作者信息

Schifferer F, Beitinger H, Rahmann H, Möbius D

机构信息

Zoological Institute, University of Stuttgart-Hohenheim, FRG.

出版信息

FEBS Lett. 1988 Jun 6;233(1):158-62. doi: 10.1016/0014-5793(88)81375-9.

DOI:10.1016/0014-5793(88)81375-9

PMID:3384088

Abstract

The influence of calcium and temperature on pure lipid (bovine brain PC, sulphatides, ganglioside GT1b), valinomycin and mixed lipid-valinomycin monolayers at the air/water interface was studied. In mixed films, evidence was found that the two components were miscible. On the other hand, at higher surface pressures, phase separation occurs in the cases of PC and sulphatides. Measuring the area requirement and the collapse pressure the stability of both lipid and the peptide was increased in particular due to ganglioside-valinomycin interaction. The addition of 10(-5) M calcium into the subphase at 20 and 37 degrees C and surface pressures of 10 and 20 mN/m led to a condensing effect in ganglioside mixtures, with formation of aggregates as indicated also by the nearly ideal behaviour of two component monolayers.

摘要

研究了钙和温度对空气/水界面处纯脂质（牛脑磷脂酰胆碱、硫脂、神经节苷脂GT1b）、缬氨霉素以及脂质-缬氨霉素混合单层膜的影响。在混合膜中，有证据表明两种组分是可混溶的。另一方面，在较高表面压力下，磷脂酰胆碱和硫脂会发生相分离。通过测量面积需求和崩塌压力发现，特别是由于神经节苷脂-缬氨霉素的相互作用，脂质和肽的稳定性均有所提高。在20℃和37℃下，当亚相中添加10⁻⁵ M钙且表面压力为10和20 mN/m时，神经节苷脂混合物会产生凝聚效应，形成聚集体，这也由两组分单层膜的近乎理想行为所表明。