• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

皂苷在前列腺癌中的抗肿瘤活性及潜在生物标志物

Anti-tumor activities of saponins and potential biomarkers in prostate cancer.

作者信息

He Shan, Lyu Fangqiao, Lou Lixia, Liu Lu, Li Songlin, Jakowitsch Johannes, Ma Yan

机构信息

Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology & Immunology, Vienna General Hospital, Medical University of Vienna, Vienna, Austria.

Department of Cell Biology, School of Basic Medicine, Capital Medical University, Beijing, China.

出版信息

J Ginseng Res. 2021 Mar;45(2):273-286. doi: 10.1016/j.jgr.2019.12.007. Epub 2020 Jan 7.

DOI:10.1016/j.jgr.2019.12.007
PMID:33841008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8020356/
Abstract

BACKGROUND

Prostate carcinoma is the second most common cancer among men worldwide. Developing new therapeutic approaches and diagnostic biomarkers for prostate cancer (PC) is a significant need. The Chinese herbal medicine saponins (PQS) have been reported to show anti-tumor effects. We hypothesized that PQS exhibits anti-cancer activity in human PC cells and we aimed to search for novel biomarkers allowing early diagnosis of PC.

METHODS

We used the human PC cell line DU145 and the prostate epithelial cell line PNT2 to perform cell viability assays, flow cytometric analysis of the cell cycle, and FACS-based apoptosis assays. Microarray-based gene expression analysis was used to display specific gene expression patterns and to search for novel biomarkers. Western blot and quantitative real-time PCR were performed to demonstrate the expression levels of multiple cancer-related genes.

RESULTS

Our data showed that PQS inhibited the viability of DU145 cells and induced cell cycle arrest at the G1 phase. A significant decrease in DU145 cell invasion and migration were observed after 24 h treatment by PQS. PQS up-regulated the expression levels of p21, p53, TMEM79, ACOXL, ETV5, and SPINT1 while it down-regulated the expression levels of bcl2, STAT3, FANCD2, DRD2, and TMPRSS2.

CONCLUSION

PQS promoted cells apoptosis and inhibited the proliferation of DU145 cells, which suggests that PQS may be effective for treating PC. TMEM79 and ACOXL were expressed significantly higher in PNT2 than in DU145 cells and could be novel biomarker candidates for PC diagnosis.

摘要

背景

前列腺癌是全球男性中第二常见的癌症。开发前列腺癌（PC）的新治疗方法和诊断生物标志物具有重大需求。据报道，中药皂苷（PQS）具有抗肿瘤作用。我们假设PQS在人PC细胞中具有抗癌活性，并且旨在寻找能够早期诊断PC的新型生物标志物。

方法

我们使用人PC细胞系DU145和前列腺上皮细胞系PNT2进行细胞活力测定、细胞周期的流式细胞术分析以及基于FACS的凋亡测定。基于微阵列的基因表达分析用于显示特定的基因表达模式并寻找新型生物标志物。进行蛋白质免疫印迹和定量实时PCR以证明多个癌症相关基因的表达水平。

结果

我们的数据表明，PQS抑制DU145细胞的活力并诱导细胞周期停滞在G1期。PQS处理24小时后，观察到DU145细胞的侵袭和迁移显著减少。PQS上调p21、p53、TMEM79、ACOX1、ETV5和SPINT1的表达水平，同时下调bcl2、STAT3、FANCD2、DRD2和TMPRSS2的表达水平。

结论

PQS促进细胞凋亡并抑制DU145细胞的增殖，这表明PQS可能对治疗PC有效。TMEM79和ACOX1在PNT2中的表达明显高于DU145细胞，可能是PC诊断的新型生物标志物候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/b7bd8bde0f17/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/8eed9833e7e5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/262916536d45/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/c6187eb69f4b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/453fc01b106e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/83ddb7f96afe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/27bd924f8f6b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/1fe65bd8cdac/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/ad8e766750c5/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/23bb14e9abb3/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/b7bd8bde0f17/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/8eed9833e7e5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/262916536d45/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/c6187eb69f4b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/453fc01b106e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/83ddb7f96afe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/27bd924f8f6b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/1fe65bd8cdac/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/ad8e766750c5/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/23bb14e9abb3/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8c5/8020356/b7bd8bde0f17/gr10.jpg

相似文献

1
Anti-tumor activities of saponins and potential biomarkers in prostate cancer.皂苷在前列腺癌中的抗肿瘤活性及潜在生物标志物
J Ginseng Res. 2021 Mar;45(2):273-286. doi: 10.1016/j.jgr.2019.12.007. Epub 2020 Jan 7.
2
Saponins (Ginsenosides) from the Leaves of Panax quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice.美洲商陆叶中的三萜皂苷（人参皂苷）可改善对乙酰氨基酚诱导的小鼠肝毒性。
J Agric Food Chem. 2017 May 10;65(18):3684-3692. doi: 10.1021/acs.jafc.7b00610. Epub 2017 Apr 25.
3
Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury.西洋参叶皂苷对顺铂诱导的急性肾损伤的肾保护作用。
Int J Mol Sci. 2017 Jul 13;18(7):1407. doi: 10.3390/ijms18071407.
4
Panax quinquefolium saponin combined with dual antiplatelet drugs inhibits platelet adhesion to injured HUVECs via PI3K/AKT and COX pathways.西洋参皂苷联合双联抗血小板药物通过PI3K/AKT和COX途径抑制血小板黏附于损伤的人脐静脉内皮细胞。
J Ethnopharmacol. 2016 Nov 4;192:10-19. doi: 10.1016/j.jep.2016.07.015. Epub 2016 Jul 8.
5
Panax quinquefolius saponin inhibits vascular smooth muscle cell calcification via activation of nuclear factor-erythroid 2-related factor 2.西洋参总皂苷通过激活核因子红细胞 2 相关因子 2 抑制血管平滑肌细胞钙化。
BMC Complement Med Ther. 2023 Apr 21;23(1):129. doi: 10.1186/s12906-023-03961-6.
6
Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro.土贝母苷甲在体外可诱导人前列腺癌细胞发生氧化应激介导的凋亡及G0/G1期阻滞。
Acta Pharmacol Sin. 2016 Jul;37(7):950-62. doi: 10.1038/aps.2016.34. Epub 2016 Jun 13.
7
Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells.白花丹醌在人前列腺癌PC-3和DU145细胞中引发差异蛋白质组学反应，主要涉及细胞周期、细胞凋亡、自噬以及上皮-间质转化途径。
Drug Des Devel Ther. 2015 Jan 7;9:349-417. doi: 10.2147/DDDT.S71677. eCollection 2015.
8
Panax quinquefolius saponin inhibits endoplasmic reticulum stress-mediated apoptosis and neurite injury and improves functional recovery in a rat spinal cord injury model.西洋参总皂苷抑制内质网应激介导的细胞凋亡和轴突损伤，改善大鼠脊髓损伤模型的功能恢复。
Biomed Pharmacother. 2018 Jun;102:212-220. doi: 10.1016/j.biopha.2018.03.074. Epub 2018 Mar 22.
9
Total saponins from stems and leaves of Panax quinquefolius L. ameliorate podophyllotoxin-induced myelosuppression and gastrointestinal toxicity.西洋参茎叶总皂苷可改善鬼臼毒素引起的骨髓抑制和胃肠道毒性。
Biomed Chromatogr. 2022 Feb;36(2):e5266. doi: 10.1002/bmc.5266. Epub 2021 Nov 12.
10
Panax quinquefolius saponins combined with dual antiplatelet drug therapy alleviate gastric mucosal injury and thrombogenesis through the COX/PG pathway in a rat model of acute myocardial infarction.西洋参总皂苷联合双联抗血小板药物治疗通过 COX/PG 通路减轻急性心肌梗死大鼠胃黏膜损伤和血栓形成。
PLoS One. 2018 Mar 27;13(3):e0194082. doi: 10.1371/journal.pone.0194082. eCollection 2018.

引用本文的文献

1
Anti-Colorectal Cancer Activity of and Its Active Components, Ginsenosides: A Review.人参及其活性成分人参皂苷的抗结直肠癌活性：综述
Int J Mol Sci. 2025 Mar 13;26(6):2593. doi: 10.3390/ijms26062593.
2
Mechanism allowing biochar to aid in arbuscular mycorrhizal colonization in Panax quinquefolius L. roots and improve secondary metabolite production.生物炭促进西洋参根系丛枝菌根定殖并提高次生代谢产物产量的机制。
Mycorrhiza. 2025 Mar 19;35(2):23. doi: 10.1007/s00572-025-01195-7.
3
The beneficial potential of ginseng for menopause.人参对更年期的潜在益处。

本文引用的文献

1
Unfavorable Pathology, Tissue Biomarkers and Genomic Tests With Clinical Implications in Prostate Cancer Management.前列腺癌管理中具有临床意义的不良病理学、组织生物标志物和基因组检测。
Adv Anat Pathol. 2018 Sep;25(5):293-303. doi: 10.1097/PAP.0000000000000192.
2
Panax quinquefolius saponins combined with dual antiplatelet drug therapy alleviate gastric mucosal injury and thrombogenesis through the COX/PG pathway in a rat model of acute myocardial infarction.西洋参总皂苷联合双联抗血小板药物治疗通过 COX/PG 通路减轻急性心肌梗死大鼠胃黏膜损伤和血栓形成。
PLoS One. 2018 Mar 27;13(3):e0194082. doi: 10.1371/journal.pone.0194082. eCollection 2018.
3
J Ginseng Res. 2024 Sep;48(5):449-453. doi: 10.1016/j.jgr.2024.05.008. Epub 2024 May 31.
4
Effect of ginseng and ginsenosides on attention deficit hyperactivity disorder: A systematic review.人参及人参皂苷对注意力缺陷多动障碍的影响：一项系统评价
J Ginseng Res. 2024 Sep;48(5):437-448. doi: 10.1016/j.jgr.2024.05.006. Epub 2024 May 28.
5
Increasing the Amounts of Bioactive Components in American Ginseng ( L.) Leaves Using Far-Infrared Irradiation.利用远红外辐射增加西洋参叶片中生物活性成分的含量
Foods. 2024 Feb 17;13(4):607. doi: 10.3390/foods13040607.
6
KRG and its major ginsenosides do not show distinct steroidogenic activities examined by the OECD test guideline 440 and 456 assays.根据经合组织测试指南440和456分析，红参及其主要人参皂苷未显示出明显的类固醇生成活性。
J Ginseng Res. 2023 May;47(3):385-389. doi: 10.1016/j.jgr.2022.09.002. Epub 2022 Oct 5.
7
Immunomodulatory, Anti-Inflammatory, and Anti-Cancer Properties of Ginseng: A Pharmacological Update.人参的免疫调节、抗炎和抗癌特性：药理学更新。
Molecules. 2023 May 3;28(9):3863. doi: 10.3390/molecules28093863.
8
Evaluation of the antioxidant and antitumor activity of extracts from leaves.叶片提取物的抗氧化和抗肿瘤活性评估
Front Chem. 2022 Oct 31;10:1035949. doi: 10.3389/fchem.2022.1035949. eCollection 2022.
9
Murr Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Modulating Tumor Microenvironment.穆尔通过调节肿瘤微环境使胃癌细胞对5-氟尿嘧啶敏感。
Front Pharmacol. 2022 May 17;13:911663. doi: 10.3389/fphar.2022.911663. eCollection 2022.
10
Lipid metabolism-related gene prognostic index (LMRGPI) reveals distinct prognosis and treatment patterns for patients with early-stage pulmonary adenocarcinoma.脂质代谢相关基因预后指数（LMRGPI）揭示了早期肺腺癌患者不同的预后和治疗模式。
Int J Med Sci. 2022 Mar 28;19(4):711-728. doi: 10.7150/ijms.71267. eCollection 2022.
Effect of Zoledronic Acid and Denosumab in Patients With Low Back Pain and Modic Change: A Proof-of-Principle Trial.
唑来膦酸和地舒单抗治疗腰痛伴 Modic 改变患者的效果：一项原理验证试验。
J Bone Miner Res. 2018 May;33(5):773-782. doi: 10.1002/jbmr.3376. Epub 2018 Feb 2.
4
Ginsenoside Rg3 inhibits colorectal tumor growth via down-regulation of C/EBPβ/NF-κB signaling.人参皂苷 Rg3 通过下调 C/EBPβ/NF-κB 信号通路抑制结直肠肿瘤生长。
Biomed Pharmacother. 2017 Dec;96:1240-1245. doi: 10.1016/j.biopha.2017.11.092. Epub 2017 Nov 21.
5
Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo.人参皂苷 Rg3 通过靶向肿瘤干细胞和肿瘤血管生成抑制结直肠癌的体内进展。
Int J Oncol. 2018 Jan;52(1):127-138. doi: 10.3892/ijo.2017.4183. Epub 2017 Nov 1.
6
Ginsenoside Rg3 enhances the anti-proliferative activity of erlotinib in pancreatic cancer cell lines by downregulation of EGFR/PI3K/Akt signaling pathway.人参皂苷 Rg3 通过下调 EGFR/PI3K/Akt 信号通路增强厄洛替尼在胰腺癌细胞系中的抗增殖活性。
Biomed Pharmacother. 2017 Dec;96:619-625. doi: 10.1016/j.biopha.2017.10.043. Epub 2017 Oct 13.
7
Ginsenoside Rg3 attenuates cisplatin resistance in lung cancer by downregulating PD-L1 and resuming immune.人参皂苷 Rg3 通过下调 PD-L1 并恢复免疫来减轻肺癌的顺铂耐药性。
Biomed Pharmacother. 2017 Dec;96:378-383. doi: 10.1016/j.biopha.2017.09.129. Epub 2017 Oct 12.
8
Interventions for managing medication-related osteonecrosis of the jaw.颌骨药物相关性骨坏死的管理干预措施。
Cochrane Database Syst Rev. 2017 Oct 6;10(10):CD012432. doi: 10.1002/14651858.CD012432.pub2.
9
Ginsenosides Rb3 and Rd reduce polyps formation while reinstate the dysbiotic gut microbiota and the intestinal microenvironment in Apc mice.人参皂苷 Rb3 和 Rd 可减少息肉形成，同时恢复 Apc 小鼠的肠道菌群失调和肠道微环境。
Sci Rep. 2017 Oct 2;7(1):12552. doi: 10.1038/s41598-017-12644-5.
10
Estradiol-ERβ2 signaling axis confers growth and migration of CRPC cells through TMPRSS2-ETV5 gene fusion.雌二醇-雌激素受体β2信号轴通过跨膜丝氨酸蛋白酶2-ETS变异体5基因融合赋予去势抵抗性前列腺癌细胞生长和迁移能力。
Oncotarget. 2016 Aug 17;8(38):62820-62833. doi: 10.18632/oncotarget.11355. eCollection 2017 Sep 8.