[组织培养中交感神经节内神经元间相互关系的特征]
[Characteristics of interneuronal interrelationships among sympathetic ganglia in tissue culture].
作者信息
Nozdrachev A D, Skopichev V G, Riakhovskaia L V, Adamatskiĭ A I
出版信息
Fiziol Zh SSSR Im I M Sechenova. 1988 Mar;74(3):350-8.
The culture of neurons of paravertebral sympathetic ganglia of 3--5 day old guinea pigs was obtained, fragments of the ganglia being implanted to adult rats-recipients. A migration of neuroblasts from the explant area, specific features of neurons differentiation with morphological signs appearing, and the growth of neurites forming specific growth cones, were revealed as well as genetically determined interneuronal connections studied in details by means of electron microscopy. The data obtained show the diffusion chambers technique to be promising for study of interneuronal connections among extramural ganglia.
获取了3 - 5日龄豚鼠椎旁交感神经节的神经元培养物，将神经节碎片植入成年大鼠受体。观察到神经母细胞从外植体区域迁移，神经元分化的特定特征以及出现形态学标志，还有形成特定生长锥的神经突生长，并且通过电子显微镜详细研究了基因决定的神经元间连接。所获得的数据表明扩散室技术在研究壁外神经节间的神经元连接方面具有前景。
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