Terebieniec Agata, Chroumpi Tania, Dilokpimol Adiphol, Aguilar-Pontes Maria Victoria, Mäkelä Miia R, de Vries Ronald P
Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands.
Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland.
Biotechnol Rep (Amst). 2021 Mar 15;30:e00610. doi: 10.1016/j.btre.2021.e00610. eCollection 2021 Jun.
d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from . This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).
D-木糖还原酶是醛糖-酮糖还原酶家族的成员之一,参与真菌中通过戊糖分解代谢途径(PCP)进行的D-木糖和L-阿拉伯糖的转化。在本研究中,我们对新鉴定出的来自……的第二种D-木糖还原酶(XyrB)进行了生化特性分析。这种依赖NADPH的还原酶能够高效转化D-木糖和L-阿拉伯糖,并且在所有目前已知的真菌戊糖还原酶中,它对这些糖类具有最高的亲和力。生化数据、转录组学和系统发育分析相结合,进一步阐明了XyrB在PCP中的作用。酶类:D-木糖还原酶(EC 1.1.1.307),L-阿拉伯糖还原酶(EC 1.1.1.21)。