Key Laboratory of Applied Marine Biotechnology of Department of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, People's Republic of China.
College of Food and Pharmaceutical Sciences, Ningbo, Zhejiang, 315211, People's Republic of China.
Bioprocess Biosyst Eng. 2021 Aug;44(8):1769-1779. doi: 10.1007/s00449-021-02559-x. Epub 2021 Apr 12.
Currently, the market price of fucoxanthin-based drugs remains high primarily because, on one hand, the main natural source of fucoxanthin, Phaeodactylum tricornutum (P. tricornutum), is extremely low in endogenous fucoxanthin, while, on the other hand, fucoxanthin mass production has proved to be very challenging. In this study, we demonstrated the feasibility of increasing fucoxanthin bioaccumulation in P. tricornutum by promoting photosynthetic activity of this diatom. Specifically, this study investigated the effects of different concentrations of the photosynthetic induction factor (PIF) on fucoxanthin content and biosynthesis, on chlorophyll fluorescence characteristics, and on the expression of photosynthesis-related genes in P. tricornutum. The results showed that the optimal PIF concentration was 1 µg L, while optimal time was 48 h, with the effect decreasing at 72 h. Fucoxanthin content increased by 44.2% compared to that of the control group in 48 h. Correlation analysis showed a significant positive correlation between fucoxanthin content and the actual photosynthetic yield of PS II (r = 0.949, P < 0.01). The total amount of energy actually used in photosystem II (PS II) by photosynthesis may be used as the main components affecting the biosynthesis of fucoxanthin in P. tricornutum. In addition, we found that using PIF to promote photosynthesis in P. tricornutum effectively increased the growth rate and bioaccumulation of fucoxanthin to an economically advantageous level, thereby providing a novel strategy for the commercial production of fucoxanthin.
目前，基于岩藻黄质的药物的市场价格仍然很高，主要原因一方面是岩藻黄质的主要天然来源三角褐指藻（Phaeodactylum tricornutum）内源岩藻黄质含量极低，另一方面是岩藻黄质的大规模生产极具挑战性。在本研究中，我们通过提高这种硅藻的光合作用活性，证明了增加三角褐指藻中岩藻黄质生物积累的可行性。具体而言，本研究调查了不同浓度的光合作用诱导因子（PIF）对岩藻黄质含量和生物合成、叶绿素荧光特性以及三角褐指藻中光合作用相关基因表达的影响。结果表明，最佳 PIF 浓度为 1μg/L，最佳时间为 48 h，72 h 后效果下降。与对照组相比，48 h 时岩藻黄质含量增加了 44.2%。相关性分析表明，岩藻黄质含量与 PS II 的实际光合产量呈显著正相关（r=0.949，P<0.01）。光合作用中 PS II 实际使用的能量总量可能是影响三角褐指藻中岩藻黄质生物合成的主要因素。此外，我们发现，使用 PIF 促进三角褐指藻的光合作用可以有效提高其生长速度和岩藻黄质的生物积累，达到经济有利的水平，从而为岩藻黄质的商业生产提供了一种新策略。
