Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, United States.
Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, United States.
Virus Res. 2021 Jul 2;299:198423. doi: 10.1016/j.virusres.2021.198423. Epub 2021 Apr 13.
The SARS coronavirus (SARS-CoV) has the potential to cause serious disease that can spread rapidly around the world. Much of our understanding of SARS-CoV pathogenesis comes from in vitro experiments. Unfortunately, in vitro experiments cannot replicate all the complexity of the in vivo infection. For example, proteases in the respiratory tract cleave the SARS-CoV surface protein to facilitate viral entry, but these proteases are not present in vitro. Unfortunately, proteases might also have an effect on other parts of the replication cycle. Here, we use mathematical modeling to estimate parameters characterizing viral replication for SARS-CoV in the presence of trypsin or elastase, and in the absence of either. In addition to increasing the infection rate, the addition of trypsin and elastase causes lengthening of the eclipse phase duration and the infectious cell lifespan.
严重急性呼吸综合征冠状病毒（SARS-CoV）有可能引起严重疾病，并在全球迅速传播。我们对 SARS-CoV 发病机制的了解主要来自体外实验。不幸的是，体外实验无法复制体内感染的所有复杂性。例如，呼吸道中的蛋白酶可裂解 SARS-CoV 表面蛋白以促进病毒进入，但这些蛋白酶在体外不存在。不幸的是，蛋白酶也可能对复制周期的其他部分产生影响。在这里，我们使用数学建模来估计在存在胰蛋白酶或弹性蛋白酶以及不存在这两种蛋白酶的情况下 SARS-CoV 复制的特征参数。除了提高感染率外，胰蛋白酶和弹性蛋白酶的添加还会导致潜伏期和感染细胞寿命延长。
