Jimoh Olatunji Abubakar, Oyeyemi Wahab Adekunle, Okin-Aminu Hafsat Ololade, Oyeyemi Bolaji Fatai
Department of Agricultural Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State, Nigeria.
Department of Physiology, Igbenedion University, Okada, Edo State, Nigeria.
Theriogenology. 2021 Jul 1;168:41-49. doi: 10.1016/j.theriogenology.2021.03.020. Epub 2021 Mar 31.
Today, orthodox medicine has almost exceeded its limits in resolving subfertility problems in animals, thus making phytomedicine a primary tool in the treatment of infertility. In this work, three herbal supplements obtained from freshly air-dried Moringa oleifera, Phyllanthus amarus and Viscum album leaves were evaluated to ascertain their comparative effect on the reproductive potentials of bucks. Sixty bucks were allotted four diets made up of standard grower rabbit ration without supplement and with 5% Moringa, Mistletoe and Phyllanthus supplementation for 84 days. Semen samples were collected from all bucks using artificial vagina, for semen quality and seminal oxidative stress markers. The organ weights, testicular and epididymal spermatozoa reserves were assessed to determine sperm production potentials using standard procedures. The result obtained revealed that spermatozoa concentration, progressive motility, curvilinear velocity, average path velocity and the amplitude of lateral head was significantly (p < 0.05) higher in the group fed with mistletoe supplemented diet compared to that recorded in the control groups. The inclusion of herbal supplements linearly (p < 0.05) increased the seminal total antioxidant activity with a corresponding decrease in the seminal lipid peroxidation across the herbal supplemented treatments compared to the control. The gonadal and extra-gonadal sperm reserves of bucks fed on the herbal supplements were depleted compared to bucks on control. Bucks on mistletoe supplementation rivalled the superior daily sperm production and testicular sperm reserve in bucks without herbal supplements. In conclusion, Viscum album supplements in bucks' diets encouraged daily sperm production, testicular sperm reserves, testosterone, as well as spermatozoa kinetics. The three herbal supplements did enhance semen oxidative stability.
如今，正统医学在解决动物繁殖力低下问题方面几乎已达到极限，因此植物医学成为治疗不孕症的主要手段。在这项研究中，对从新鲜风干的辣木、余甘子和槲寄生叶中提取的三种草药补充剂进行了评估，以确定它们对雄兔繁殖潜力的比较效果。将60只雄兔分配到四种日粮组，日粮分别由不添加补充剂的标准生长兔日粮以及添加5%辣木、槲寄生和余甘子的日粮组成，持续84天。使用人工阴道从所有雄兔采集精液样本，检测精液质量和精液氧化应激标志物。采用标准程序评估器官重量、睾丸和附睾精子储备，以确定精子生产潜力。结果显示，与对照组相比，饲喂添加槲寄生日粮组的精子浓度、前向运动率、曲线速度、平均路径速度和头部侧摆幅度显著更高（p < 0.05）。与对照组相比，添加草药补充剂线性增加了精液总抗氧化活性（p < 0.05），同时相应降低了各草药补充处理组的精液脂质过氧化水平。与对照组雄兔相比，饲喂草药补充剂的雄兔性腺和性腺外精子储备减少。添加槲寄生的雄兔在每日精子产量和睾丸精子储备方面可与未添加草药补充剂的雄兔相媲美。总之，在雄兔日粮中添加槲寄生可促进每日精子生成、睾丸精子储备、睾酮分泌以及精子运动学。这三种草药补充剂确实提高了精液的氧化稳定性。
